Chloride Transport Pathways in Human Keratinocytes
The transport of C1- across cell membrane plays an important role in a number of biological functions, including the maintenance of cell volume and the regulation of intracellular pH. A variety of systems capable of transporting C1- across the plasma membrane have been described in mammalian cells. They include the electroneutral C1- /anion exchange and C1-/cation cotrans-port, and the electrically conductive Cl- channels. The Cl-/ anion exchange has been shown to be involved in regulation of intracellular pH in hamster fibroblasts (L’Allemain et al., 1985) and in Vero cells (Olsnes et al., 1986). In some cell types, it has been reported that the Cl-/cation cotransport can be activated by cell shrinkage induced by hypertonic media in a process named regulatory volume increase (for a review see Hoffmann, 1986). Conversely, in human lymphocytes (Grinstein et al., 1982), Ehrlich ascites tumor cells (Hoffmann, 1986), and human fibroblasts (Rugolo et al., 1989), cell swelling induced by hypotonic shock has been clearly demonstrated to activate electrically conductive Cl- channels, in a process named regulatory volume decrease.
KeywordsCystic Fibrosis Anion Exchange Human Keratinocytes Regulatory Volume Decrease Ehrlich Ascites Tumor Cell
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