Regulation of Ion Conductance in Human Skin Fibroblasts
The function of the CFTR (Cystic Fibrosis Transport Regulatory) protein is unknown but based on ion flux and electrophysiological data in epithelial cells it appears probable that this protein functions either directly or indirectly in the regulation of ion transport. Current hypotheses suggest that this protein may be more specifically related to the activation of chloride channels via protein kinase A or C mediated phosphorylation since abnormal chloride channel regulation by these enzymes has been observed in airway epithelial cells obtained from cystic fibrosis patients (1,2,3). However, conductance pathways for other ions, ie. sodium ion, may also be affected in cystic fibrosis (4). In an attempt to further define the role for the CFTR protein we tested the hypothesis that cell types which express only low levels of CFTR, i.e. nonepithelial cells, will fail to exhibit one or more important features of ion transport regulation known to exist in epithelial cells. As cyclic AMP and calcium mediated ion conductance has been described quite well in epithelial cells we assessed the role of these regulatory pathways in ion conductance in skin fibroblasts.
KeywordsCystic Fibrosis Chloride Channel Human Skin Fibroblast Single Channel Activity Single Channel Opening
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