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Purification of the Epithelial Cl Channel

  • Qais Al-Awqati
  • Donald Landry
  • Myles Akabas
  • Christopher Redhead
  • Aleksander Edelman
  • John Edwards
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 290)

Abstract

Chloride channels are present in plasma membranes and in intracellular organelles. In a variety of epithelial cells these channels mediate NaCl absorption and secretion. The aim of our work was to purify the Cl channel and to develop molecular reagents that could be used to study the biochemical basis of cystic fibrosis.

Keywords

Cystic Fibrosis Chloride Channel Parafollicular Cell Soybean Phospholipid Cetic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    D.W. Landry, M. Reitman, E.J. Cragoe and Q. Al-Awqati, 1987, Epithelial Chloride Channels; Development of inhibitory ligands, J. Gen. Physiol., 90:779.PubMedCrossRefGoogle Scholar
  2. 2.
    D.W. Landry, M.H. Akabas, C.Redhead, A. Edelman, E.J. Cragoe, Jr., and Q. Al-Awqati, 1989, Purification and reconstitution of chloride channels from kidney and trachea, Scien ce, 244:1469.69.Google Scholar
  3. 3.
    J. Barasch, M.D. Gershon, E.A. Nunez, H. Tamir and Q. AlAwqati, 1988, Thyrotropin induces the acidification of the secretory granules of parafollicular cells by increasing the chloride conductance of the granular membrane,J. Cell Biol., 107:2137.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • Qais Al-Awqati
    • 1
  • Donald Landry
    • 1
  • Myles Akabas
    • 1
  • Christopher Redhead
    • 1
  • Aleksander Edelman
    • 1
  • John Edwards
    • 1
  1. 1.Departments of Medicine and Physiology, College of Physicians and SurgeonsColumbia UniversityNew YorkUSA

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