High Resolution NMR Studies of Cerebral Glucose Metabolism in Rats and Humans
During the past four years we have been studying human brains by NMR methods that we developed first in animal experiments. These methods allow us to obtain 1H and 13C NMR spectra from the brain while we also apply 31P NMR methods such as are generally used in the field. The first 1H NMR spectra of the brain, or in fact of any living animal tissue, were of the rat brain at high magnetic fields of 8.4T (1). To obtain these spectra it was necessary to suppress the intense 1H NMR peak from H2O in order to observe the peaks from metabolites. This was done, at first by pre-saturation of the H2O peak and later by selective pulses that did not excite the H2O peak (2) . Subsequently Rothman introduced editing methods into proton spectroscopy of the brain to simplify the spectrum. First homonuclear editing was used (3) and then heteronuclear in which 13C labeled nuclei were determined from their coupled protons C4.
KeywordsHigh Magnetic Field Cerebral Glucose Metabolism Selective Pulse Couple Proton Proton Spectroscopy
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