Rous Sarcoma Virus Gag Gene Sequences Affect Viral RNA Splicing and Stability

  • Mark T. McNally
  • George F. Barker
  • Karen Beemon
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)


A feature common to the life cycles of all retroviruses is the incomplete splicing of the primary viral RNA transcript. While splicing of a subset of the primary transcripts is necessary to generate subgenomic mRNAs (including env mRNA and additional mRNAs in the more complex retroviruses), a major fraction of the RNA transcripts are transported to the cytoplasm in an unspliced form, where they serve both as mRNA and as genomic RNA (Coffin, 1990; Stoltzfus, 1989).


Splice Acceptor Site Triosephosphate Isomerase Rous Sarcoma Virus Nonsense Codon Field Virology 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Arrigo, S., and Beemon, K., 1988, Regulation of Rous sarcoma virus RNA splicing and stability, Mol. Cell. Biol., 8: 4858.PubMedGoogle Scholar
  2. Arrigo, S., Yun, M. and Beemon, K., 1987, cis-acting regulatory elements within the gag genes of avian retroviruses, Mol. Cell. Biol., 7: 388.PubMedGoogle Scholar
  3. Bolognesi, D.P., Montelaro, R.C., Frank, H., and Schafer, W., 1978, Assembly of type C oncornaviruses: a model, Science. 199: 183.PubMedCrossRefGoogle Scholar
  4. Cann, A.J., and Chen, I.S.Y., 1990, Human T-cell leukemia virus types I and II, In: “Fields Virology”, 2nd ed., B.N. Fields and D.M. Knipe, ed., Raven Press, N.Y., N.Y.Google Scholar
  5. Cheng, J., Fogel-Petrovic, M., and Maquat, L.E., 1990, Translation to near the distal end of the penultimate exon is required for normal levels of spliced triosephosphate isomerase mRNA, Mol. Cell. Biol., 10: 5215.PubMedGoogle Scholar
  6. Coffin, J.M., 1990, Retroviridae and their replication, In: “Fields Virology”, 2nd ed., B.N. Fields and D.M. Knipe, ed., Raven Press, N.Y., N.Y.Google Scholar
  7. Hiraga, S., and Yanofsky, C., 1972, Hyper-labile messenger RNA in polar mutants of the tryptophan operon of Escherichia coli, J. Mol. Biol., 72: 103.Google Scholar
  8. Katz, R. A., Kotler, M. and Skalka, A. M., 1988, cis-acting intron mutations that affect the efficiency of avian retroviral RNA splicing: implications for mechanisms of control, J. Virol. 62:2686.PubMedGoogle Scholar
  9. Katz, R. A., and Skalka, A. M., 1990, Control of retroviral RNA splicing through maintenance of suboptimal processing signals, Mol. Cell. Biol., 10: 696.PubMedGoogle Scholar
  10. Schwartz, D.E., Tizard, R., and Gilbert, W., 1983, Nucleotide sequence of Rous sarcoma virus, Cell, 32: 853.PubMedCrossRefGoogle Scholar
  11. Stoker, A.W., and Bissell, M.J., 1988, Development of avian sarcoma and leukosis virus-based vector-packaging cell lines, J. Virol., 62: 1008.PubMedGoogle Scholar
  12. Stoltzfus, C. M., 1989, Synthesis and processing of avian sarcoma retrovirus RNA, Adv. Virus. Res. 63: 1.Google Scholar
  13. Stoltzfus, C. M., and Fogarty, S. J., 1989, Multiple regions in the Rous sarcoma virus src gene intron act in cis to affect the accumulation of unspliced RNA, J. Virol., 63: 1669.PubMedGoogle Scholar
  14. Stoltzfus, C. M., Lorenzen, S. K., and Berberich, S. L., 1987, Noncoding region between the env and src genes of Rous sarcoma virus influences splicing efficiency at the src gene 3’ splice site, J. Virol., 61:177.PubMedGoogle Scholar
  15. Urlaub, G., Mitchell, P.J., Ciudad, C.J., and Chasin, L.A., 1989, Nonsense mutations in the dihydrofolate reductase gene affect RNA processing, Mol. Cell. Biol. 9: 2868.PubMedGoogle Scholar
  16. Wong-Staal, F., 1990, Human immunodeficiency viruses and their replication, In: “Fields Virology”, 2nd ed., B.N. Fields and D.M. Knipe, ed., Raven Press, N.Y., N.Y.Google Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Mark T. McNally
    • 1
  • George F. Barker
    • 1
  • Karen Beemon
    • 1
  1. 1.Department of BiologyJohns Hopkins UniversityBaltimoreUSA

Personalised recommendations