DNA Cleaving Activity of Purified Human Immunodeficiency Virus Integration Protein

  • Paula A. Sherman
  • James A. Fyfe
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)


An essential step in the life cycle of retroviruses is insertion of a double-stranded DNA copy of the viral RNA genome into the host cell DNA, to form the provirus. The insertion event depends on at least one viral protein, the integration protein (IN), which is a product of the viral pol gene. Mutations in the IN coding region of pol result in integration-negative retroviruses that will no longer replicate.1–3 The proviral DNA is identical to the precursor viral DNA except for the loss of two base pairs at each end, at the points of attachment to cellular DNA. One proposed function for IN is the removal of these two bases from the 3′-termini of both strands of the viral DNA, in preparation for integration.


Minus Strand Avian Myeloblastosis Virus Supercoiled Plasmid Oligonucleotide Substrate Supercoiled Plasmid pBR322 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    P. Schwartzberg, J. Colicelli, and S. P. Goff, Construction and analysis of deletion mutations in the pol gene of Maloney murine leukemia virus: a new viral function required for productive infection, Cell 37: 1043 (1984).PubMedCrossRefGoogle Scholar
  2. 2.
    L. Donehower and H. E. Varmus, A mutant murine leukemia virus with a single missense codon in pol is defective in a function affecting integration, Proc. Natl. Acad. Sci. USA 81:6461 (1984).PubMedCrossRefGoogle Scholar
  3. 3.
    A. T. Panganiban and H. M. Temin, The retrovirus pol gene encodes a product required for DNA integration: identification of a retrovirus int locus, Proc. Natl. Acad. Sci. USA 81:7885 (1984).PubMedCrossRefGoogle Scholar
  4. 4.
    P. A. Sherman and J. A. Fyfe, Human immunodeficiency virus integration protein expressed in Escherichia coli possesses selective DNA cleaving activity, Proc. Natl. Acad. Sci. USA 87:5119 (1990).PubMedCrossRefGoogle Scholar
  5. 5.
    D. P. Grandgenett, A. C. Vora, and R. D. Schiff, A 32,00-dalton nucleic acid-binding protein from avian retrovirus cores possesses DNA endonuclease activity, Virology 89:119 (1978).PubMedCrossRefGoogle Scholar
  6. 6.
    J. Leis, G. Duyk, S. Johnson, M. Longiaru, and A. Skalka, Mechanism of action of the endonuclease associated with the ap and pp forms of avian RNA tumor virus reverse transcriptase, J. Virol. 45:727 (1983).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Paula A. Sherman
    • 1
  • James A. Fyfe
    • 1
  1. 1.Wellcome Research LaboratoriesResearch Triangle ParkUSA

Personalised recommendations