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Roles of Nucleocapsid Cysteine Arrays in Retroviral Assembly and Replication: Possible Mechanisms in RNA Encapsidation

  • Robert J. Gorelick
  • Stephen M. NigidaJr.
  • Larry O. Arthur
  • Louis E. Henderson
  • Alan Rein
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)

Summary

The nucleocapsid (NC) proteins of all retroviruses contain either one or two copies of a sequence motif, C-X2-C-X4-H-X4-C, which has been termed the “cysteine array” or Cys-His box. Studies from several laboratories have shown that mutants in this motif direct the production of virus particles; these particles are structurally normal in many respects, but are either partially or completely deficient in genomic RNA. Further, even when some RNA is encapsidated, the particles are almost completely noninfectious.

We review some of the known properties of these particles and of particles lacking genomic RNA for other reasons. Based on these properties, we propose that only dimeric RNA is encapsidated, and that the cysteine array(s) are involved in a positive selection or “search” for this dimeric RNA during virus assembly. Further speculations on the nature of the protein-RNA interactions in RNA packaging are also presented.

Keywords

Human Immunodeficiency Virus Type Murine Leukemia Virus Virus Assembly Equine Infectious Anemia Virus Rous Sarcoma Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Robert J. Gorelick
    • 1
  • Stephen M. NigidaJr.
    • 1
  • Larry O. Arthur
    • 1
  • Louis E. Henderson
    • 1
  • Alan Rein
    • 2
  1. 1.AIDS Vaccine ProgramPRI/DynCorpFrederickUSA
  2. 2.Laboratory of Molecular Virology and Carcinogenesis, ABL-Basic Research ProgramNCI-Frederick Cancer Research and Development CenterFrederickUSA

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