Mechanistic Analysis of HIV-1 Reverse Transcriptase

  • John Abbotts
  • Samuel H. Wilson
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)


Two biological issues have formed the basis for this laboratory’s investigation of the reverse transcriptase (RT) of human immunodeficiency virus, type 1 (HIV-1). The first issue lies with the fact that the reverse transcriptase is an obvious target for drug therapy against HIV (Mitsuya and Broder, 1987). The approach in this regard has been to identify fundamental characteristics of the enzyme, with the idea that such information may facilitate rational drug design. We describe here work to identify the kinetic features of the HIV reverse transcriptase, including studies employing as substrate the triphosphates of the inhibitor AZT and one of its analogues.


Reverse Transcriptase Product Molecule Avian Myeloblastosis Virus Avian Myeloblastosis Virus Reverse Transcriptase Polypeptide Species 
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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • John Abbotts
    • 1
  • Samuel H. Wilson
    • 1
  1. 1.Laboratory of BiochemistryNational Cancer Institute, National Institutes of HealthBethesdaUSA

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