Mechanistic Analysis of HIV-1 Reverse Transcriptase
Two biological issues have formed the basis for this laboratory’s investigation of the reverse transcriptase (RT) of human immunodeficiency virus, type 1 (HIV-1). The first issue lies with the fact that the reverse transcriptase is an obvious target for drug therapy against HIV (Mitsuya and Broder, 1987). The approach in this regard has been to identify fundamental characteristics of the enzyme, with the idea that such information may facilitate rational drug design. We describe here work to identify the kinetic features of the HIV reverse transcriptase, including studies employing as substrate the triphosphates of the inhibitor AZT and one of its analogues.
KeywordsReverse Transcriptase Product Molecule Avian Myeloblastosis Virus Avian Myeloblastosis Virus Reverse Transcriptase Polypeptide Species
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