t-RNA Genes in Streptomyces Lividans: Structure, Organization and Construction of Suppressor t-RNA

  • Reinhard Sedlmeier
  • Horst Schmieger
Part of the Federation of European Microbiological Societies Symposium Series book series (FEMS, volume 55)


The successful study of bacteriophages require a collection of various conditionally lethal mutants, preferably nonsense mutants. Nonsense mutants have a mutationally caused stop codon inside a reading frame which causes the premature termination of a peptide chain in translation. Nonsense mutants, on the other hand, can only be obtained and handled if bacterial suppressor strains are available. Such strains are characterized by a mutated t-RNA which has an alteration in the anticodon enabling it to insert an aminoacid at the nonsense codon position. Therefore, the first step in starting genetics with a phage is the search for suitable suppressor strains.


Codon Usage tRNA Gene Nonsense Mutant Synonymous Codon Bias Codon Usage 
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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Reinhard Sedlmeier
    • 1
  • Horst Schmieger
    • 1
  1. 1.Institut für Genetik und MikrobiologieUniversität MünchenMünchen 19Germany

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