t-RNA Genes in Streptomyces Lividans: Structure, Organization and Construction of Suppressor t-RNA
The successful study of bacteriophages require a collection of various conditionally lethal mutants, preferably nonsense mutants. Nonsense mutants have a mutationally caused stop codon inside a reading frame which causes the premature termination of a peptide chain in translation. Nonsense mutants, on the other hand, can only be obtained and handled if bacterial suppressor strains are available. Such strains are characterized by a mutated t-RNA which has an alteration in the anticodon enabling it to insert an aminoacid at the nonsense codon position. Therefore, the first step in starting genetics with a phage is the search for suitable suppressor strains.
KeywordsCodon Usage tRNA Gene Nonsense Mutant Synonymous Codon Bias Codon Usage
Unable to display preview. Download preview PDF.
- Ikemura, T., 1981, Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes: A proposal for a synonymous codon choice that is optimal for the E. coli translational system. J. Mol. Biol., 151: 389–409.PubMedCrossRefGoogle Scholar
- Sedlmeier, R., 1987, Charakterisierung einer Quecksilber-Resistenz aus Streptomyces lividans. Dipl. Thesis, Regensburg.Google Scholar