DNA Topoisomerases from Streptomyces and Their Inhibition by Some Antibiotic and Antitumoractive Agents

  • K. Störl
  • J. Störl
  • Ch. Zimmer
Part of the Federation of European Microbiological Societies Symposium Series book series (FEMS, volume 55)


It has been recognized that DNA supercoiling is an essential factor in the maintainan a of the chromosomal state and cellular growth of bacteria.1–3 Supercoiling plays an important role in the interaction with a large number of proteins and hence influences the cellular processes such as replication, recombination, transcription and repair. In prokaryotes, DNA supercoiling is controlled by the action of two enzymes, DNA gyrase and DNA topoisomerase I. Gyrase, a type II topoisomerase produces negative DNA supercoils and can remove negative as well as positive supercoils.1–3 Topoisomerase I relaxes negatively supercoiled DNA and counteracts the supercoiling activity of gyrase.2,3 Studies on DNA topoisomerases, their enzymatic properties and inhibitory effects of various agents have been reported for different microorganisms.1–4 Topoisomerases from E.coli are the most extensively investigated enzymes.1,3,5


Minor Groove Oxolinic Acid Gyrase Inhibitor Positive Supercoils Streptomyces Noursei 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    J. C. Wang, DNA topoisomerases, Annu. Rev. Biochem. 54: 665 (1985).PubMedCrossRefGoogle Scholar
  2. 2.
    K. Drlica, Biology of bacterial DNA topoisomerases, Microbiol. Rev. 48: 273 (1984)PubMedGoogle Scholar
  3. 3.
    Ch. Zimmer, K. Störl, and J. Störl, Microbial DNA topoisomerases and their inhibition by antibiotics, J.Basic Microbiol. in press (1990).Google Scholar
  4. 4.
    K. Drlica and S. Coughlin, Inhibitors of DNA gyrase, Pharmac. Ther. 44: 107 (1989).CrossRefGoogle Scholar
  5. 5.
    M. Gellert, DNA topoisomerases, Annu. Rev. Biochem. 50: 879 (1981).PubMedCrossRefGoogle Scholar
  6. 6.
    O. G. Stonington and D. E. Pettijohn, The folded genome of Escherischia coli isolated in a protein-DNA-RNA complex, Proc. Natl. Acad. Sci. USA 68: 6 (1971).PubMedCrossRefGoogle Scholar
  7. 7.
    E. Sarfert, Ch. Zimmer, J. Gumpert, and H. Lang, Folded chromosome structure and DNA-binding protein of Streptomyces hygroscopicus, Biochim. Biophys. Acta 740: 118 (1983).Google Scholar
  8. 8.
    E. Sarfert, V. Sedova, H. Triebel, H. Bär, and Ch. Zimmer, DNA binding protein from Streptomyces hygroscopicus: detection of binding by gel retardation, sedimentation and effects on the transcriptional activity in vitro, Biomed. Biochim. Acta 9: 633 (1989).Google Scholar
  9. 9.
    K. Störl, J. Störl, Ch. Zimmer, M. Roth, and D. Noack, Isolation and properties of DNA topoisomerasel and DNA gyrase from Streptomyces noursei, J. Basic Microbiol. in press (1990).Google Scholar
  10. 10.
    H.-P. Voosberg, DNA topoisomerases; enzymes that control DNA conformation, Curr. Top. Microbiol. Immunnl., 114: 19 (1985).CrossRefGoogle Scholar
  11. 11.
    L. L. Shen and A. G. Pernet, Mechanism of inhibition of DNA gyrase by analogues of nalidixic acid: The target of the drugs is DNA, Proc. Natl. Acad. Sci. USA 82: 307 (1985).PubMedCrossRefGoogle Scholar
  12. 12.
    L. L. Shen, L. A. Mitscher, P. N. Sharma, T. J. O’Donnell, D. W. T. Chu, C. S. Cooper, T. Rosen, and A. G Pernet, Mechanism of inhibition of DNA gyrase by quinolone antibacterials: A cooperative drug- DNA binding model, Biochemistry 28: 3886 (1989).PubMedCrossRefGoogle Scholar
  13. 13.
    M. M. Zweerink, and A. Edison, Inhibition of Micrococcus luteus DNA gyrase by norfloxacin and 10 other quinolone carboxylic acids, Antimicrob. Agents Chemother. 29: 598 (1986).PubMedGoogle Scholar
  14. 14.
    X. Taaary, N. Moreau, C. Dureuil, and F. Le Goffic, Effect of DNA gyrase inhibitors pefloxacin, five other quinolones, novobiocin and chlorobiocin on E. coli topoisomerasel, Antimicrob. Agents Chemother. 31: 1925 (1987).Google Scholar
  15. 15.
    Ch. Zimmer and U. Wâhnert, Nonintercalating DNA -binding ligands: Specificity of the interaction and their use as tools in biophysical, biochemical and biological investigations of the genetic material, Proo. Biophys. Molec. Biol. 47: 31 (1986).CrossRefGoogle Scholar
  16. 16.
    D. C. Straney and D. M. Crothers, Effect of drug-DNA interactions upon transcription initiation at the lac promoter. Biochemistry 26: 1987 (1987).PubMedCrossRefGoogle Scholar
  17. 17.
    J. M. Woynarowski, R. D. Sigmund, and T. A. Beerman, DNA minor groove binding agents interfere with topoisomeraseII mediated lesions induced by epipodophyllotoxin derivative VM-26 and acridine derivative m-AMSA in nuclei from L1210 cells, Biochemistry 28: 3850 (1989).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • K. Störl
    • 1
  • J. Störl
    • 1
  • Ch. Zimmer
    • 1
  1. 1.Central Institute of Microbiology and Experimental TherapyAcademy of Sciences of the GDRJenaGDR

Personalised recommendations