Construction and Use of a Secretion Vector in Streptomyces

  • Claudine Piron-Fraipont
  • Mauro V. Lenzini
  • Jean Dusart
Part of the Federation of European Microbiological Societies Symposium Series book series (FEMS, volume 55)


The main research project of the Fundamental Bacteriology Unit in Liège is the unravelling, at the molecular level, of the mode of action of β-lactam antibiotics. For this purpose, most of the model target enzymes have been selected in the genus Streptomyces (Frère and Joris, 1985; Ghuysen et al., 1989). In the frame of this program, we were brought along to overproduce several of these model enzymes as well as several β-lactamases. This was carried out by cloning the corresponding genes in Streptomyces multicopy plasmids as pIJ702 or related vectors. This part of the work is now over, seven genes (three β-lactamases, and four penicillin-sensitive DD-peptidases) have been cloned, and sequenced and their products were overexpressed in Streptomyces lividans (Dehottay et al., 1986, 1987; Duez et al., 1987 and unpublished results; Lenzini et al., 1987, 1988; Piron-Fraipont et al., 1989; Houba et al., 1989).


BglII Site Secretion Vector Condary Structure 
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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Claudine Piron-Fraipont
    • 1
  • Mauro V. Lenzini
    • 1
  • Jean Dusart
    • 1
  1. 1.Fundamental Bacteriology Unit, Department of MicrobiologyUniversity of LiègeLiègeBelgium

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