Construction and Use of a Secretion Vector in Streptomyces
The main research project of the Fundamental Bacteriology Unit in Liège is the unravelling, at the molecular level, of the mode of action of β-lactam antibiotics. For this purpose, most of the model target enzymes have been selected in the genus Streptomyces (Frère and Joris, 1985; Ghuysen et al., 1989). In the frame of this program, we were brought along to overproduce several of these model enzymes as well as several β-lactamases. This was carried out by cloning the corresponding genes in Streptomyces multicopy plasmids as pIJ702 or related vectors. This part of the work is now over, seven genes (three β-lactamases, and four penicillin-sensitive DD-peptidases) have been cloned, and sequenced and their products were overexpressed in Streptomyces lividans (Dehottay et al., 1986, 1987; Duez et al., 1987 and unpublished results; Lenzini et al., 1987, 1988; Piron-Fraipont et al., 1989; Houba et al., 1989).
KeywordsBglII Site Secretion Vector Condary Structure
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