Pullulan-Hydrolyzing Enzymes of Streptomycetes: Effect of Carbon Source on Their Production and Characterization of a Pullulanase-Negative Mutant

  • Frank Hänel
  • Hans Krügel
  • Theo Peschke
Part of the Federation of European Microbiological Societies Symposium Series book series (FEMS, volume 55)


Streptomycetes are Gram-positive mycelial soil bacteria that obtain nutrients from macromolecular organic debris by using a wide range of hydrolytic enzymes. These enzymes include proteases, nucleases, lipases, and a variety of enzymes that hydrolyze different types of often quite complex polysaccharides (Williams et al., 1983). These include α-amylases (1,4-α--D-glucan-4-glucanohydrolase, EC that are responsible for the breakdown of starch by the endoglucanic cleavage of 1,4-α--linked glucose residues (Guilbot and Mercier, 1985) and pullulanases (α--dextrin endo-l,6-α-glucosidase, EC, which hydrolyze α-(1--6)-glucosidic linkages of starch and pullulan (Bender and Wallenfels, 1961).α-Amylases of Streptomyces have received considerable recent attention (Gräfe et al., 1986; McKillop et al., 1986; Hoshiko et al., 1987; Virolle and Bibb, 1988; Virolle et al., 1988). In contrast, there are only a few reports on pullulanases of streptomycetes (Ueda et al., 1971; Yagisawa et al., 1972). In addition to their importance as industrial enzymes, pullulanases are also of interest to basic research. For example, no data are available concerning a possible induction of pullulanase genes by small oligosaccharides derived from starch or pullulan (e.g. malto-triose) or repression by more readily assimilated sugars(e.g. glucose) in streptomycetes.


Sole Carbon Source Amylase Activity Aerial Mycelium Streptomyces Griseus Glucosidase Activity 


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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Frank Hänel
    • 1
  • Hans Krügel
    • 1
  • Theo Peschke
    • 1
  1. 1.Central Institute of Microbiology and Experimental TherapyGDR Academy of SciencesJenaDeutschland

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