Investigations of Expression and Protein Sequence of the Aminoacetyltransferase Gene Nat1 from Streptomyces Noursei
Nourseothricin (Nc) belongs to the group of streptothricin (St) antibiotics that includes grisin and racemomycin, which exhibit antibacterial, antifungal and antiviral activity (Bocker and Bergter, 1986). Although they are not used therapeutically they give remarkable effects as fodder additives in animal husbandry (Bocker and Bergter, 1986). Resistance against the streptothricins is mediated via acetylation (Keeratipibul, 1983, Tschaepe et al., 1984, Haupt et al., 1986). From NMR studies Kobayashi et al. (1987) found that in Streptomyces lavendulae this involves a monoacetylation of the beta-amino-group of the beta-lysine moiety of the streptothricin. The gene (stat) for this enzymatic activity was cloned and sequenced and its transcription start shown to be near the translational start (Horinouchi et al., 1987). In addition, a transposon-specified St-acetyltransferase (SAT) was identified in enterobacteria isolated from animals treated with Nc (Tschaepe et al., 1984). The nucleotide sequence of this gene was determined by Hein et al. (1989). From Streptomyces noursei a gene (nat)1 conferring resistanc to Nc via acetylation was cloned as plasmid pNAT1 and analyzed (Krügel et al., 1988).
KeywordsStreptomyces Noursei Aminoglycoside Phosphotransferase Inverted Repeat Structure Perfect Inverted Repeat Fodder Additive
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