Abstract
Tetracycline antibiotics and their semisynthetic derivatives are now being widely used clinically and veterinarily. Industrial strains — the producers of oxy- and chlortetracycline (Otc and Ctc) are the objects of long-term conventional selection and possess high level of antibiotic activity. In recent years considerable success has been met in research on genetic control of Otc biosynthesis in Streptomyces rimosus strains. All genes for Otc biosynthesis are localized on one long DNA fragment of about 34 kb1,2,8. The genes for biosynthesis are flanked by genes responsible for resistance to Otc, otrA and otrB, “early” biosynthesis genes being linked to otrB and “late” genes to otrA. Coordinated transcription of resistance and biosynthesis genes has been demonstrated.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Binnie C., Warren M., Butler M. (1989) Cloning and heterologous expression in Streptomyces lividans of Streptomyces rimosus genes involved in oxytetracycline biosynthesis. J. Bacteriol. 171: 887–895.
Butler M.J., Friend E.J., Hunter I.S., Kaczmarek F.S., Sugden D.A., Warren M. (1989) Molecular cloning of resistance genes and architecture of the linked gene cluster involved in biosynthesis of oxytetracycline by Streptomyces rimosus. Mol.Gen.Genet. 215: 231–238.
Chinenova T.A., Biryukova I.V., Voeykova T.A., Emelyanova L.K. Klochkova 0.A., Sezonov G.V., Lomovskaya N.D. (1989)Identification of determinants for resistance to chlortetracycline and other antibiotics in S. aureofaciencs strain — the producer of chlortetracycline. Genetika (USSR) 26: 00.
Hinterman G., Grarneri R., Kieser T., Hutter R. (1981) Restriction analysis of the Streptomyces glaucescens genome by agarose gel electrophoresis. Arch. Microbiol. 131: 218–222.
Hopwood D.A., Bibb M.J., Chater K.F.,Kieser T., Bruton C.J., Kieser H.M., Lydiate D.J., Smith C.F., Ward J.M., Schrempf H. (1985) Genetic manipulation of Streptomyces: a laboratory manual. John Innes Foundation, Norwich, 355 p.
Hopwood D.A., Kieser T., Wright H.M., Bibb M.J. (1983) Plasmids, recombination and chromosome mapping in Streptomyces lividans 66. J. Gen. Microbiol. 129: 2257–2269
Hostalek Z., Vanek Z. (1985) Biosynthesis of the tetracyclines. In: Handbook Experim. Pharmacol. 78: P. 137.
Hunter I.S., Deidre D., McDowall K.J. (1990) Molecular genetics of oxytetracycline production in Streptomyces rimosus J. Cell. Biochem., Abstracts 19th Annual Meetings UCLA Symposia on Mol. Cell. Biol. CC012, p. 91
Kessler A., Dittrich W., Betzler M., Schrempf H. (1989) Cloning and analysis of a deletable tetracycline-resistance determinant of Streptomyces lividans 1326. Molecular Microbiology. 3 (8): 1103–1109.
Maniatis T., Fritsch E.E., Sambrook J. (1982) Molecular cloninng. A laboratory manual.Cold Spring Harbor Lab. P. 480
Mc.Cormick I.R.D. (1969) Point-blocked mutants and, the biogenesis f. tetracyclines. In: Sermonti G., Alacevic M. (eds). Genetics and breeding of Streptomyces. Yugoslavian Academy of Sciences and Arts. Zagreb, P. 163.
Ohnuki T., Katoh T., Imanaka T., Aiba S. (1985) Molecular cloning of tetracycline resistance genes from Streptomyces rimosus into S.griseus and characterization of the cloned genes. J.Bacteriol. 161: 1010–1016.
Reunes J.P., Calmels T., Drocourt D., Tiraby G. (1988) Cloning,expression in Escherichia coli and nucleotid sequense of a tetracyclin-resistance gene from Streptomyces rimosus. J.Gen.Microbiol. 134: 585–598
Zvenigorodsky V.I., Lomovskaya N.D. (1972) Induction of prophage by a virulent mutant of actinophage фC31 Streptomyces coelicolor A3(2). Genetika (USSR) 8: 94–100.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Plenum Press, New York
About this chapter
Cite this chapter
Lomovskaya, N., Sezonov, G., Isayeva, L., Chinenova, T. (1991). Genetic Characterization and Cloning of Genes for Chlortetracycline Resistance in Streptomyces Aureofaciens Strains. In: Baumberg, S., Krügel, H., Noack, D. (eds) Genetics and Product Formation in Streptomyces . Federation of European Microbiological Societies Symposium Series, vol 55. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5922-7_14
Download citation
DOI: https://doi.org/10.1007/978-1-4684-5922-7_14
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5924-1
Online ISBN: 978-1-4684-5922-7
eBook Packages: Springer Book Archive