Genetic Characterization and Cloning of Genes for Chlortetracycline Resistance in Streptomyces Aureofaciens Strains

  • Natalie Lomovskaya
  • Gennady Sezonov
  • Lyubov Isayeva
  • Tatyana Chinenova
Part of the Federation of European Microbiological Societies Symposium Series book series (FEMS, volume 55)


Tetracycline antibiotics and their semisynthetic derivatives are now being widely used clinically and veterinarily. Industrial strains — the producers of oxy- and chlortetracycline (Otc and Ctc) are the objects of long-term conventional selection and possess high level of antibiotic activity. In recent years considerable success has been met in research on genetic control of Otc biosynthesis in Streptomyces rimosus strains. All genes for Otc biosynthesis are localized on one long DNA fragment of about 34 kb1,2,8. The genes for biosynthesis are flanked by genes responsible for resistance to Otc, otrA and otrB, “early” biosynthesis genes being linked to otrB and “late” genes to otrA. Coordinated transcription of resistance and biosynthesis genes has been demonstrated.


Inducible Gene Biosynthesis Gene Macrolide Antibiotic Streptomyces Strain Divergent Line 
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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Natalie Lomovskaya
    • 1
  • Gennady Sezonov
    • 1
  • Lyubov Isayeva
    • 1
  • Tatyana Chinenova
    • 1
  1. 1.Institute of Genetics and Selection of Industrial MicroorganismsMoscowUSSR

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