Characterization of Eight Groups (Species) of Saccharopolyspora Phages — An Example for Studying Modular Organization of Actinophage Genomes

  • Jörg Schneider
Part of the Federation of European Microbiological Societies Symposium Series book series (FEMS, volume 55)


27 actinophages of Saccharopolyspora (formerly Faenia) rectivirgula, Sap. hirsuta, Sap. hordei and Sap. erythraea were compared with respect to host range, genome length and G+C composition, virion morphology and DNA homology. According to the rules of the ICTV it was thus possible to allocate the phages to 8 new bacteriophage species. Interspecific DNA hybridization indicated a modular organization of the phage genomes: some DNA regions, presumably modules, are distributed independently among many of the phage species described. For further studies of such modules different approaches were chosen: (1) the A-“module” was used to integrate a thiostrepton resistance gene (tsr) by homologous recombination into the prophages of øFR113 and øFR755R to be able to monitor module exchange in future studies. (2) Several virulent mutants of the temperate øFRb-D have lost the same short DNA region; the corresponding fragments of the wild-type phage, a deletion mutant and a virulent mutant without deletions were cloned for further analysis. (3) The restriction maps of øFRb-D and øFRb-P are presented; both phages are closely related but øFRb-P has a broader host-range among Saccharoployspora than øFRb-D.


Sugar Cane Bagasse Virulent Mutant Bacteriophage Genome Modular Organization BglII Site 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Ackermann H-W, Dubow MS (1987). Viruses of Procaryotes. Boca Raton, Florida, CRC Press.Google Scholar
  2. Botstein D (1980). A theory of modular evolution for bacteriophages. Ann NY Acad Sci 354, 484–491.PubMedCrossRefGoogle Scholar
  3. Braun V, Hertwig S, Neve H, Geis A, Teuber M (1989). Taxonomic differentiation of bacteriophages of Lactococcus lactis by electron microscopy, DNA-DNA hybridization, and protein profiles. J Gen Microbiol 135, 2551–2560.Google Scholar
  4. Brzezinski R, Surmacz E, Kutner M, Piekarowicz A (1986). Restriction mapping and close relationship of the DNA of Streptomyces erythraeus phages 121 and SE-5. J Gen Microbiol 132, 2937–2943.PubMedGoogle Scholar
  5. Campbell A, Botstein D (1983). Evolution of lambdoid phages. In Lambda II. RW Hendrix, JW Roberts, FW Stahl, RA Weisberg, eds., Cold Spring Harbor, New York.Google Scholar
  6. Chater KF (1986). Streptomyces phages and their application to Streptomyces genetics. In Antibiotic-producing Streptomyces. SW Queener, LE Day, eds., Academic Press, London.Google Scholar
  7. Donadio S, Paladino R, Costanzi I, Sparapani P, Schreil W, Iaccarino M (1986). Characterization of bacteriophages infecting Streptomyces erythreus and properties of phage-resistant mutants. J Bacteriol 166, 1055–1060.PubMedGoogle Scholar
  8. Embley TM, Smida J, Stackebrandt E (1888). The phylogeny of mycolateless wall chemotype IV actinomycetes and description of Pseudonocardiaceae fam. nov. J Syst Appt Microbiol 11, 44–52.CrossRefGoogle Scholar
  9. Gayer-Herkert G, Schneider J, Kutzner HJ (1989). Transfection and transformation of protoplasts of the thermophilic actinomycete Faenia rectivirgula. Appt Microbiol Biotechnol 31, 371–375.Google Scholar
  10. Goodfellow M, Lacey J, Athalye M, Embley TM, Bowen T (1989). Saccharopolyspora gregorii and Sap. hordei: two new actinomycete species from fodder. J Gen Microbiol 135, 2125–2139.Google Scholar
  11. Grund AD, Hutchinson CR (1987). Bacteriophages of Saccharopolyspora erythraea. J Bacteriol 169, 3013–3022.PubMedGoogle Scholar
  12. Haroutunian SJ, Kajoian SV, Karabekov BP, Reulets MA, Khrenova EA, Akhverdian VZ, Krylov VN (1986). Comparative studies of bacteriophages specific for Brevibacterium flavum. Biotechnologia 6, 21–27.Google Scholar
  13. Kamp D (1987). The evolution of Mu. In Phage Mu. N Symonds, A Toussaint, P Van De Putte, MM Howe, eds., Cold Spring Harbor, New York.Google Scholar
  14. Katz L, Chiang S-JD, Tuan JS, Zablen LB (1988). Characterization of bacteriophage 0069 of Saccharopolyspora erythraea and demonstration of heterologous actinophage propagation by transfection of Streptomyces and Saccharopolyspora. J Gen Microbiol 134, 1765–1771.PubMedGoogle Scholar
  15. Kempf A, Greiner-Mai E, Schneider J, Korn-Wendisch F, Kutzner HJ (1987). A group of actinophages of Faenia rect. Curr Microbiol 15, 283–285.CrossRefGoogle Scholar
  16. Korn-Wendisch F, Kempf A, Grund E, Kroppenstedt RM, Kutzner HJ (1989). Transfer of Faenia rectivirgula Kurup and Agre 1983 to the genus Saccharopolyspora Lacey and Goodfellow 1975, elevation of Sap. hirsuta subsp. taberi Labeda 1987 to species level, and emended description of the genus Saccharopolyspora. Int J Syst Bacteriol 39, 430–441.CrossRefGoogle Scholar
  17. Krylov VN, Akhverdyan VZ, Bogush VG, Khrenova EA, Reulets MA (1985). The modular structure of transposable phage genomes of Pseudomonas aeruginosa. Genetika 21, 724–734.Google Scholar
  18. Labeda DP (1987). Transfer of the type strain of Streptomyces erythraeus (Waksman 1923) Waksman and Henrici 1948 to the genus Saccharopolyspora Lacey and Goodfellow 1975 as Sap. erythraea sp. nov. and designation of a neotype strain for Streptomyces erythraeus. Int J Syst Bacteriol 37, 19–22.CrossRefGoogle Scholar
  19. Lacey G, Goodfellow M (1975). A novel actinomycete from sugar cane bagasse Saccharopolyspora hirsuta Error! Hyperlink reference not valid. sp. nov. J Gen Microbiol 88, 75–85.PubMedGoogle Scholar
  20. Matthews REF (1982). Classification and nomenclature of viruses. Fourth Report of the International Committee on Taxonomy of Viruses. Intervirol 17, 1–200.CrossRefGoogle Scholar
  21. Prauser H, Momirova S (1970). Phagensensitivität, Zellwandzusammensetzung und Taxonomie einiger thermophiler Actinomyceten. Zentralbl Allg Mikrobiol 10, 219–222.CrossRefGoogle Scholar
  22. Schneider J, Aguilera-Garcia I, Kutzner HJ (1987). Characterization of a family of temperate actinophages of Faenia rectivirgula. J Gen Microbiol 133, 2263–2268.Google Scholar
  23. Schneider J, Kutzner HJ (1989a). Distribution of modules among the central regions of the genomes of several actinophages of Faenia and Saccharopolyspora. J Gen Microbiol 135, 1671–1678.Google Scholar
  24. Schneider J, Kutzner HJ (1989b). Distribution of homologies among the genomes of several actinophages of Faenia and Saccharopolyspora as determined by DNA hybridization. Intervirol 30, 237–240.Google Scholar
  25. Schneider J, Kramer D, Grund E, Kutzner HJ (1989). Preliminary characterization of a group of actinophages of the thermophilic actinomycete genus Saccharomonospora. Intervirol 30, 323–329.Google Scholar
  26. Schneider J, Korn-Wendisch F, Kutzner HJ (1990). 0SC623, a temperate actinophage of Streptomyces coelicolor Müller, and its relatives 0SC347 and 0SC681. J Gen Microbiol 136, in press.Google Scholar
  27. Sinclair RB, Bibb MJ (1988). The repressor gene (c) of the Streptomyces temperate phage 0c31: Nucleotide sequence, analysis and functional cloning. Mol Gen Genet 213, 269–277.PubMedCrossRefGoogle Scholar
  28. Smorawinska M, Denis F, Dery C, Magny P, Brzezinski R (1988). Characterization of SE-3, a virulent bacteriophage of Saccharopolyspora erythraea. J Gen Microbiol 134, 1773–1778.Google Scholar
  29. Sonnen H, Schneider J, Kutzner HJ (1990a). Characterization of 0GA1, an inducible phage particle from Brevibacterium flavum. J Gen Microbiol 136, 567–571.Google Scholar
  30. Sonnen H, Schneider J, Kutzner, HJ (1990b). Corynephage Cog, a virulent bacteriophage of Corynebacterium glutamicum,and its relation to 0GA1. J Gen Virol 71,in press.Google Scholar
  31. Susskind MM, Botstein D (1978). Molecular genetics of bacteriophage P22. Microbiol Rev 42, 385–413.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Jörg Schneider
    • 1
  1. 1.Angewandte Genetik der MikroorganismenFB Biologie/Chemie der Universität OsnabrückOsnabrückGermany

Personalised recommendations