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Characterization of Eight Groups (Species) of Saccharopolyspora Phages — An Example for Studying Modular Organization of Actinophage Genomes

  • Jörg Schneider
Part of the Federation of European Microbiological Societies Symposium Series book series (FEMS, volume 55)

Summary

27 actinophages of Saccharopolyspora (formerly Faenia) rectivirgula, Sap. hirsuta, Sap. hordei and Sap. erythraea were compared with respect to host range, genome length and G+C composition, virion morphology and DNA homology. According to the rules of the ICTV it was thus possible to allocate the phages to 8 new bacteriophage species. Interspecific DNA hybridization indicated a modular organization of the phage genomes: some DNA regions, presumably modules, are distributed independently among many of the phage species described. For further studies of such modules different approaches were chosen: (1) the A-“module” was used to integrate a thiostrepton resistance gene (tsr) by homologous recombination into the prophages of øFR113 and øFR755R to be able to monitor module exchange in future studies. (2) Several virulent mutants of the temperate øFRb-D have lost the same short DNA region; the corresponding fragments of the wild-type phage, a deletion mutant and a virulent mutant without deletions were cloned for further analysis. (3) The restriction maps of øFRb-D and øFRb-P are presented; both phages are closely related but øFRb-P has a broader host-range among Saccharoployspora than øFRb-D.

Keywords

Sugar Cane Bagasse Virulent Mutant Bacteriophage Genome Modular Organization BglII Site 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Jörg Schneider
    • 1
  1. 1.Angewandte Genetik der MikroorganismenFB Biologie/Chemie der Universität OsnabrückOsnabrückGermany

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