Is Testicular Function in Immature Rats Increased Rather Than Decreased by a Moderate Increase in Temperature?

  • Anders R. J. Bergh
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 286)

Abstract

Spermatogenesis cannot be maintained at normal body temperature, but the cellular mechanisms responsible are unknown. The most common experimental approach to study this is to examine the effects of experimental cryptorchidism or heat application in adult animals. Experimental cryptorchidism is generally obtained by returning a mature descended testis back into the abdomen. This approach has been used in a great number of studies and the general conclusion is that it results in a rapid degeneration of early spermatids and spermatocytes (van Demark and Free, 1970; Setchell, 1978). It has been suggested that these particular cell types are directly sensitive to increased temperature in vitro, possibly by effects on their cell membranes (Lee and Fritz, 1973) or on their protein synthesis (Nakamura et al., 1978). When experimental cryptorchidism is induced in newborn pigs, it eventually results in decreases in testis weight, number of gonocytes and in total Leydig cell mass (identical changes as in congenital cryptorchid pigs; van Straaten, 1978), suggesting that cell types other than developed germ cells are sensitive to increased temperature.

Keywords

Cholesterol Lactate Androgen Estradiol Plasminogen 

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References

  1. Bergh, A. (1981) Morphological signs of a direct effect of experimental cryptorchidism on the Sertoli cells in rats irradiated as fetuses.Biol. Reprod. 24: 145.PubMedCrossRefGoogle Scholar
  2. Bergh, A. (1983a) Early morphological changes in the abdominal testes in immature unilaterally cryptorchid rats.Int. J. Androl. 6: 73.PubMedCrossRefGoogle Scholar
  3. Bergh, A. (1983b) Paracrine regulation of Leydig cells by the seminiferous tubules.Int. J. Androl. 6: 57.PubMedCrossRefGoogle Scholar
  4. Bergh, A. (1989) Experimental models of cryptorchidism. In: The Cryptorchid Testis. Eds: Abney, T.O. and Keel, B.A. CRC Press, Boca Raton, p 15.Google Scholar
  5. Bergh, A., Helander, H.F. and Wahlquist, L. (1978) Studies on factors governing testicular descent in the rat, particularly the role of the gubernaculum testis.Int. J. Androl. 1: 342.CrossRefGoogle Scholar
  6. Bergh, A., Damber, J-E., Ritzén, E.M. (1984) Early signs of Sertoli and Leydig cell dysfunction in the abdominal testis in immature unilaterally cryptorchid rats.Int. J. Androl. 7: 389.CrossRefGoogle Scholar
  7. Bergh, A., Damber, J-E., Huhtaniemi, I. (1987a) Intratesticular steroids and gonadotropin receptor concentrations in the testes of immature unilaterally cryptorchid rats.Int. J. Androl. 10: 803.PubMedCrossRefGoogle Scholar
  8. Bergh, A., Damber, J-E., Jacobsson, B.H., Nilsson, T.K. (1987b) Production of lactate and tissue plasminogen activator in vitro by seminiferous tubules obtained from unilaterally cryptorchid rats.Arch. Androl. 19: 177.PubMedCrossRefGoogle Scholar
  9. Gonzales, G.F., Risbridger, G.P., de Kretser, D.M. (1989) In vivo and in vitro production of inhibin by cryptorchid testes from adult rats.Endocrinology124: 1661.PubMedCrossRefGoogle Scholar
  10. Hagenäs, L., Ritzén, E.M., Svensson, J., Hansson, V. (1978) Temperature dependence of Sertoli cell function.Int. J. Androl. Suppl 2: 449.CrossRefGoogle Scholar
  11. Hoffmann, A-M., Bergh, A., Olivecrona, T. (1988) Changes of testicular cholesteryl ester hydrolase activity in experimental cryptorchid rats.J. Reprod. Fert. 86: 11.CrossRefGoogle Scholar
  12. Jean, C. (1973) Croissance et structure des testicules cryptorchides chez les souris nees de meres traitees a l’oestradiol pendant la gestation.Ann. Endocr. (Paris) 34: 669.Google Scholar
  13. Lee, L.P. and Fritz, I.B. (1973) Studies on spermatogenesis in the rat. V. Increased thermal lability of lysosomes from testicular germ cells and its possible relationship to impairment in spermatogenesis in cryptorchidism.J. Biol. Chem. 247: 7956.Google Scholar
  14. Nakamura, M., Romrell, L.J. and Hall, P.F. (1978) Influence of temperature and glucose on protein biosynthesis by immature (round) spermatids.J. Cell. Biol. 79:1.PubMedCrossRefGoogle Scholar
  15. Rommerts, F.F., de Jong, F.H., Grootegoed, J.A. and van der Molen, H.J. (1980) Metabolic changes in testicular cells from rats after long-term exposure to 37°C in vivo or in vitro.J. Endocr. 85: 471.PubMedCrossRefGoogle Scholar
  16. Setchell, B.P. (1978) The Mammalian Testis. Paul Elek, London, p 360.Google Scholar
  17. Steinberger, A. (1981) Regulation of inhibin secretion in the testis. In: Intragonadal Regulation of Reproduction. Eds: Franchimont, P. and Channing, C.P. New York, Academic Press, p 283.Google Scholar
  18. van Demark, N.L. and Free, M.J. (1970) Temperature effects. In: The Testis, vol III. Eds: Johnson, A.D., Gomes, W.R. and van Demark, N.L. Academic Press, New York, p 233.Google Scholar
  19. van Straaten, H.W.M. (1978) Lack of a primary defect in maldescended testis of the neonatal pig.Biol. Reprod. 19: 994.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Anders R. J. Bergh
    • 1
  1. 1.Department of PathologyUniversity of UmeåUmeåSweden

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