Identification of Antigens Specific to Germ-Tubes of Candida Albicans by Monoclonal Antibodies
Various ligands have been involved in the process of infection and tissue invasion of C. albicans 1. It is adherence is enhanced when germ-tubes are emerging from blastospores. The importance of yeast germination raises the possibility of a molecular organization of cell walls different between blastospores and hyphae. Major biochemical differences have been described,but the functional significance of the molecules specific to germ-tubes remains to be determined. Further features should be given by the hybridoma technology to identify surface antigens. Paradoxically a limited number of informative monoclonal antibodies was obtained by immunization of mice with whole organisms. Generally mannoproteins of yeasts cell wall were recognized 2,5. A different approach was attempted from fibrinogen binding factors released by the germ-tubes of C. albicans that we had reported previously 1. We describe the reactivity and characteristics of a murine monoclonal antibody 3D9 directed to a component of germ-tube of C. albicans.
KeywordsGerm Tube Yeast Cell Wall Cell Wall Extract High Molecular Weight Component Chronic Mucocutaneous Candidiasis
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