Advertisement

Isolation of Candida Albicans Histidine Auxotroph (HIS 4) and Characterization of Its Etiologic Gene

  • S. Gottlieb
  • E. Segal
  • G. Lebens
  • I. Polacheck
  • Z. Altboum
Part of the Federation of European Microbiological Societies Symposium Series book series (FEMS, volume 50)

Abstract

The clinical importance of C. albicans requires the development of a genetic system in this organism for better understanding of its mechanism(s) of pathogenesis and to eventually develop improved therapies for candidal infection. Our previous studies focused on the attachment of the fungus to host mammalian tissues1–3. Those studies involved a specific C. albicans isolate-CBS562. We considered it important to develop a genetic system in this strain. Consequently, we describe herewith the isolation of an histidine auxotroph (his4), and isolation, characterization and chromosomal location of the homologous HIS4 gene.

Keywords

Genetic System Chromosomal Band Candidal Infection Tetracycline Resistance Gene Lithium Acetate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    E. Segal, CRC Crit. Rev. Microhiol., 14, 229 (1987).Google Scholar
  2. 2.
    E. Segal, Microhiol. Sci., 4: 344 (1987).Google Scholar
  3. 3.
    H. Sandovsky-Losica, E. Segal in: “Proceedings of FEMS-Symposium on Candida and Candidamycosis”, E.Tümbay, H.P.R. Seeliger and Ö. Ang, eds., Plenum Publishing House, London (1990).Google Scholar
  4. 4.
    F.R. Fink, Genetics, 53: 445 (1966).PubMedGoogle Scholar
  5. 5.
    A.Rosenbluh et al., Mol. Gen. Genet., 200: 500 (1985).PubMedCrossRefGoogle Scholar
  6. 6.
    F. Sherman, rlethods in Yeast Genetics “, Gold Spring Harbor Laboratory, Cold Spring Harbor, N.Y. (1972).Google Scholar
  7. 7.
    I. Polacheck and G.A. Lebens, J. Gen. Microhiol., 135: 65 (1989).Google Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • S. Gottlieb
    • 1
  • E. Segal
    • 1
  • G. Lebens
    • 2
  • I. Polacheck
    • 2
  • Z. Altboum
    • 3
  1. 1.Department of Human Microbiology, Sackler School of MedicineTel Aviv UniversityTel AvivIsrael
  2. 2.Department of Clinical MicrobiologyHadassah Medical CenterJerusalemIsrael
  3. 3.Department of MicrobiologyIsrael Institute of Biological ResearchNess-ZionaIsrael

Personalised recommendations