Inositol 1,4,5-Trisphosphate Activates Ca2+ Channels in the Plasma Membranes of Rat Brain Nerve Terminals
There is accumulating evidence that a wide variety of stimulation of receptors by hormones and neurotransmitters results in increased phosphoinositide turnover and mobilization of Ca2+ from intracellular stores (Berridge and Irvine, 1989). Such post receptor mechanisms are relevant to the stimulation of phospholipase C-mediated hydrolysis of phosphatidylinositol 4,5-bisphosphate giving rise to diacylglycerol and to inositol 1,4,5-trisphosphate (IP3). Furthermore, IP3 mobilizes Ca2+ from microsomal organelles such as rough-and smooth-endoplasmic reticulum and calsiosome in various secretory cells (Henne et al., 1987; Payne and Fein, 1987; Volpe et al., 1988; see a review by Adbel-Latif, 1986). Thus, it is likely that the IP3-induced calcium mobilization from intracellular organelles is involved in hormone secretion by receptor stimulation.
KeywordsSynaptic Plasma Membrane Fractional Release Inositol Trisphosphate Receptor Islet Activate Protein Intact Synaptosome
Unable to display preview. Download preview PDF.
- Fabiato, A. and Fabiato, F., 1979, Calculator programs for computing the composition of the solutions cocntaining multiple metals and ligands used for experiments in skinned muscle cells, J. Physiol. (Paris), 75:463– 505.Google Scholar
- Gray, E. G. and Whittaker, V. P., 1962, The isolation of nerve endings from brain: an electronmicroscopic study of fragments derived from homogenization and centrifugation, J. Anat. (Lond.), 96:79–86.Google Scholar
- Verma, A. K., Filoteo, A. G., Stanford, D. R., Wieben, E. D., Penniston, J. T., Strehler, E. E., Fischer, R., Heim, R., Vogel, G., Mathews, S., Strehler-Page, M.-A., James, P., Vorherr, T., Krebs, J. and Carafoli, E., 1988, Complete primary structure of a human plasma membrane Ca2+ pump, J. Biol. Chem., 263:14152–14159.PubMedGoogle Scholar
- Yoshihara, Y., Ueda, H., Fujii, N., Shide, A., Yajima, H. and Satoh, M., 1990, Purification of a novel type of calcium-activated neutral protease from rat brain -possible involvement in production of the neuropeptide, kyotorphin, from calpastatin fragments, J. Biol. Chem. (in press).Google Scholar