The Differential Expression Pattern of the mRNAs Encoding β Subunits (β1 , β2 and β3) of GABAA Receptor in Rat Brain
Gamma-aminobutyric acid (GABA) is a well known major inhibitory neurotransmitter in the vertebrate brain. Its inhibitory action is mediated through the activation of the specific receptors: the GABAA and GABAB receptors. Binding of GABA with GABAA receptor (GABAA-R) causes the chloride channel to open (for reviews see Roberts, 1986; Stephenson, 1988). Recent molecular cloning analysis has revealed that GABAA-R in the brain has at least five subunits and each subunit is consisted of several variants: α 1–5, β 1–3, γ 1–2, δ, and ε (Schofield et al., 1987; Levi tan et al., 1988; Richards et al., 1989; Schofield, 1989; Shivers et al., 1989; Ymer et al., 1989a, b). On the other hand, there is much evidence suggesting the heterogeneity of GABAA-R in the brain. For example, using specific oligonucleotide probes for α 1, α 2 or α 3 subunit mRNAs, Wisden et al. (1988) have revealed that the neurons expressing α 1 and α 2 subunit mRNAs are most abundant in layers II–IV of the bovine frontal cortex, while those expressing α 3 subunit mRNA are most abundant in layers V and VI. In addition, recent investigation by Ymer et al.
KeywordsGABAA Receptor Globus Pallidus Vestibular Nucleus Piriform Cortex Subunit mRNA
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