Expression of G-Protein α-Subunit cDNA
A family of membrane-associated G-proteins are essential for receptor-effector coupling (Gilman, 1987; Neer and Clapham, 1988; Ross, 1989). G-proteins are composed of three subunits termed α,βPand γ. The α-subunit contains a binding site for guanine nucleotides and possesses the GTPase activity. It is believed that the GTP-bound α-subunit is an active form for the effector system and the functional life time is determined by the GTPase activity. Molecular cloning of G-protein α-subunit (Gα) has revealed the existence of nine or more Gα genes and a high degree of amino acid sequence homology. However, it is not clear whether each Gα transduces a distinct signal or not.
KeywordsAdenylate Cyclase Pertussis Toxin Stimulate Adenylate Cyclase Cardiac Calcium Channel Early Gene Promoter
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- Harris-Warrick, R. M., Hammond, C., Paupardin-Tritsch, D., Homburger, V., Rouot, B., Bockaert, J. and Gerschenfeld, H. M. (1988). An α40 subunit of a GTP-binding protein immunologically related to Go mediates a dopamine-induced decrease of Ca2+ current in snail neurons. Neuron 1, 27–32.PubMedCrossRefGoogle Scholar
- Itoh, H., Kozasa, T., Nagata, S., Nakamura, S., Katada, T., Ui, M., Iwai, S., Ohtsuka, E., Kawasaki, H., Suzuki, K.,and Kaziro, Y. (1986). Molecular cloning and sequence determination of cDNAs for α subunits of the guanine nucleotide-binding proteins Gs, Gi, and Go from rat brain. Proc. Natl. Acad. Sci. USA 83, 3776–3780.PubMedCrossRefGoogle Scholar
- Nukada, T., Tanabe, T., Takahashi, H., Noda, M., Haga, K., Haga, T., Ichiyama, A., Kangawa, K., Hiranaga, M., Matsuo, H. and Numa, S. (1986) . Primary structure of the α-subunit of bovine adenylate cyclase-inhibiting G-protein deduced from the cDNA sequence. FEBS Lett. 197. 305–310.PubMedCrossRefGoogle Scholar
- Tanabe, T., Nukada, T., Nishikawa, Y., Sugimoto, K., Suzuki, H., Takahashi, H., Noda, M., Haga, T., Ichiyama, A., Kangawa, K., Minamino, N., Matsuo, H. and Numa, S. (1985). Primary structure of the α-subunit of transducin and its relationship to ras proteins. Nature 315, 242–245.PubMedCrossRefGoogle Scholar