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Expression of G-Protein α-Subunit cDNA

  • Toshihide Nukada
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 287)

Abstract

A family of membrane-associated G-proteins are essential for receptor-effector coupling (Gilman, 1987; Neer and Clapham, 1988; Ross, 1989). G-proteins are composed of three subunits termed α,βPand γ. The α-subunit contains a binding site for guanine nucleotides and possesses the GTPase activity. It is believed that the GTP-bound α-subunit is an active form for the effector system and the functional life time is determined by the GTPase activity. Molecular cloning of G-protein α-subunit (Gα) has revealed the existence of nine or more Gα genes and a high degree of amino acid sequence homology. However, it is not clear whether each Gα transduces a distinct signal or not.

Keywords

Adenylate Cyclase Pertussis Toxin Stimulate Adenylate Cyclase Cardiac Calcium Channel Early Gene Promoter 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Toshihide Nukada
    • 1
    • 2
  1. 1.Department of Medical ChemistryKyoto University Faculty of MedicineKyoto 606Japan
  2. 2.Department of Biochemistry Institute of Brain Reseach, Faculty of MedicineUniversity of TokyoTokyo 113Japan

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