Expression of G-Protein α-Subunit cDNA

  • Toshihide Nukada
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 287)


A family of membrane-associated G-proteins are essential for receptor-effector coupling (Gilman, 1987; Neer and Clapham, 1988; Ross, 1989). G-proteins are composed of three subunits termed α,βPand γ. The α-subunit contains a binding site for guanine nucleotides and possesses the GTPase activity. It is believed that the GTP-bound α-subunit is an active form for the effector system and the functional life time is determined by the GTPase activity. Molecular cloning of G-protein α-subunit (Gα) has revealed the existence of nine or more Gα genes and a high degree of amino acid sequence homology. However, it is not clear whether each Gα transduces a distinct signal or not.


Adenylate Cyclase Pertussis Toxin Stimulate Adenylate Cyclase Cardiac Calcium Channel Early Gene Promoter 
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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Toshihide Nukada
    • 1
    • 2
  1. 1.Department of Medical ChemistryKyoto University Faculty of MedicineKyoto 606Japan
  2. 2.Department of Biochemistry Institute of Brain Reseach, Faculty of MedicineUniversity of TokyoTokyo 113Japan

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