Nutritional Regulation of Apolipoprotein Genes: Effect of Dietary Carbohydrates and Fatty Acids

  • Agnès Ribciro
  • Marise Mangeney
  • Philippe Cardot
  • Claude Loriette
  • Jean Chambaz
  • Yves Rayssiguier
  • Gilbert Béréziat
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 285)


The effect of nutritional factors on apolipoprotein gene expression by rat liver were studied. Dietary carbohydrates or fatty acids regulate the expression of apo E gene, by altering either gene transcription or mRNA stability. Conversely, apo AT regulation occurs at a post transcriptional level. In vivo and in vitro experiments gave contradictory results concerning apo B gene expression. The more dramatic changes in plasma lipids and apolipoproteins are obtained under dietary fish oil. Hepatocytes from fish oil-fed rats retain for several days modification in fatty acid metabolism, i.e. a shift in oleic acid channeling towards oxidation at the expense of csterification and a reduced ability to synthesize and secrete triacylglycerol. These modifications are paralleled with a decrease in the synthesis and in the secretion of apo Bs. Hepatocytes from fish oil fed rats secrete degradative forms of apo B which might result from either a sluggish VLDL synthesis and secretion or a more specific effect of n-3 long chain polyunsaturated fatty acid peroxidative products. Hepatocytes from fish oil fed rats exhibit a reduced ability to synthesize cholesterol, associated with a decrease in apo AT synthesis and secretion without any modification in apo AI mRNA. In contrast, the hepatocytes exhibit a concomitent decrease in apo E synthesis and secretion and in cellular apo E mRNA levels.


Oleic Acid Essential Fatty Acid Dietary Carbohydrate Essential Fatty Acid Deficiency Plasma Triacylglycerol 


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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Agnès Ribciro
    • 1
  • Marise Mangeney
    • 1
  • Philippe Cardot
    • 2
  • Claude Loriette
    • 1
  • Jean Chambaz
    • 1
  • Yves Rayssiguier
    • 2
  • Gilbert Béréziat
    • 1
  1. 1.Laboratoire de biochimie (CNRS URA 1283) CHU Saint-AntoineUniversité Pierre et Marie CurieParisFrance
  2. 2.Laboratoire des maladies métaboliquesINRATheixFrance

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