Regression of Atheroma and Putative Role of CETP in Cholesteryl Ester Removal
Evidence for regression of atherosclerosis induced by cholesterol feeding has been provided by several investigators [1–3]. However, since the extent of atherosclerotic involvement is quite variable, quantitative evaluation of regression is difficult. We have used 3H-cholesteryl linoleyl ether (3H-CLE), a nonhydrolyzable analog of cholesteryl easter as a stable marker for the quantitation of atherosclerotic involvement  and evaluated the potential usefulness of 3H-CLE in the evaluation of regression of atheromatosis . To that end, 20 rabbits were kept on a purina diet enriched with 1% cholesterol for 1 month and then on alternate weeks for an additional 2 months. The animals were randomized into two groups according to their plasma cholesterol levels and injected with autologous plasma labeled with 3H-CLE . The baseline group was killed 10–12 days after injection, while the regression group was fed purina fortified with 3% cholestyramine and killed 8–11 months after injection of the 3H-CLE. We investigated the following: 1. Will the 3H-CLE remain in the aorta during the 11-month period of regression? 2. If 3H-CLE is retained, then the specific activity expressed as 3H-CLE/CE mass should rise with CE loss during regression; 3. Is the loss of CE during regression similar from the different parts of the aorta?
KeywordsCholesteryl Ester Regression Group Baseline Group Cholesterol Feeding Atherosclerosis Regression
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