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Regression of Atheroma and Putative Role of CETP in Cholesteryl Ester Removal

  • Yechezkiel Stein
  • Olga Stein
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 285)

Abstract

Evidence for regression of atherosclerosis induced by cholesterol feeding has been provided by several investigators [1–3]. However, since the extent of atherosclerotic involvement is quite variable, quantitative evaluation of regression is difficult. We have used 3H-cholesteryl linoleyl ether (3H-CLE), a nonhydrolyzable analog of cholesteryl easter as a stable marker for the quantitation of atherosclerotic involvement [4] and evaluated the potential usefulness of 3H-CLE in the evaluation of regression of atheromatosis [5]. To that end, 20 rabbits were kept on a purina diet enriched with 1% cholesterol for 1 month and then on alternate weeks for an additional 2 months. The animals were randomized into two groups according to their plasma cholesterol levels and injected with autologous plasma labeled with 3H-CLE [5]. The baseline group was killed 10–12 days after injection, while the regression group was fed purina fortified with 3% cholestyramine and killed 8–11 months after injection of the 3H-CLE. We investigated the following: 1. Will the 3H-CLE remain in the aorta during the 11-month period of regression? 2. If 3H-CLE is retained, then the specific activity expressed as 3H-CLE/CE mass should rise with CE loss during regression; 3. Is the loss of CE during regression similar from the different parts of the aorta?

Keywords

Cholesteryl Ester Regression Group Baseline Group Cholesterol Feeding Atherosclerosis Regression 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Yechezkiel Stein
    • 1
    • 2
  • Olga Stein
    • 1
    • 2
  1. 1.Lipid Research Laboratory, Department of Medicine BHadassah University HospitalJerusalemIsrael
  2. 2.Department of Experimental Medicine and Cancer ResearchHebrew University-Hadassah Medical SchoolJerusalemIsrael

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