Purification and Properties of Baboon Corneal Aldehyde Dehydrogenase: Proposed UVR Protective Role
The mammalian cornea plays an important role in protecting the eye from ultra-violet radiation (UVR) induced damage, particularly photosensitive retinal cells and the lens, by the absorption of UVR in the 290–320 nm wavelength range (UV-B) (Boettner and Wolters, 1962; Zigman, 1983). The mechanisms of corneal photoreception of UV-B light have not been determined, as yet, although studies examining the action spectra for the cornea in the induction of photokeratitis, have suggested the major involvement of a soluble protein in this process (Cogan and Kinsey, 1946; see Ringvold, 1980) . Photobiological processes in the cornea, arising from UV-B absorption, have also not been described. Recent studies by Stephens et al (1989) have indicated, however, that lipid peroxidation may account for phototoxicity in the retina, via the generation of peroxidic aldehydes, which are the cytotoxic products of lipid peroxidation (see Esterbauer et al, 1988).
KeywordsAldehyde Dehydrogenase Papio Hamadryas Percent Acetic Acid Corneal Extract Bovine Enzyme
Unable to display preview. Download preview PDF.
- Abedinia, M., Pain, T., Algar, E.M. and Holmes, R.S., 1990, Bovine corneal aldehyde dehydrogenase: the major soluble corneal protein with a possible dual protective role for the eye. Exp. Eye Research (in press).Google Scholar
- Algar, E.M. and Holmes, R.S., 1989b, Kinetic properties of murine liver aldehyde dehydrogenases, in “Enzymology and Molecular Biology at Carbonyl Metabolism” 2, H.Weiner and T.G. Flynn, eds., Alan R. Liss, N.Y., 93.Google Scholar
- Boettner, E.A. and Wolters, J.R., 1962, Transmittance of the ocular media, Invest. Ophthalmol., 1:776.Google Scholar
- Esterbauer, H. Zollner, H. and Schaur, R.J., 1988, Hydroxyalkenals:cytotoxic products of lipid peroxidation, ISI Atlas of Science; Biochemistry, l:3ll.Google Scholar
- Koivula, T. and Koivusalo, M., 1975, Different forms of rat liver aldehyde dehydrogenase and their subcellular distribution, Biochim, Biophys. Acta, 397:9.Google Scholar
- Ringvold, A., 1980, Cornea and ultraviolet radiation, Acta Ophthalmol. 58:63.Google Scholar
- Stephens, R.J., Negi, D.S., Short, S.M. van Kuijk, J.G.M., Dratz, E.A. and Thomas, D.W., 1989, lipid peroxidation and retinal phototoxic degeneration, in Oxygen Radicals in Biology and Medicine, M.G. Simic, K.A. Taylor, J.F. Ward and C. von Sonntag, eds., Plenum, N.Y., :283.,Google Scholar