Reactivity of Enzyme Modification Reagents with Aldose Reductase and Aldehyde Reductase

  • Tadashi Mizoguchi
  • Hiroyuki Itabe
  • Peter F. Kador
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 284)


Aldose reductase (EC and aldehyde reductase (EC are members of a broad family of NADPH-dependent reductases (Feldstead and Bachur, 1980). While the physiological role of either enzyme remains unknown, it is generally assumed that aldose reductase reduces aldose sugars to their respective sugar alcohols while aldehyde reductase reduces a broad range of aromatic and aliphatic aldehydes. Aldehyde reductase is also known as hexonate dehydrogenase EC because of its ability to oxidize L-gulonic acid to glucuronic acid..


Aldose Reductase Aldose Reductase Inhibitor Desalt Column Carbonyl Reductase Aldehyde Reductase 
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  1. Bohren, K.M., Bullock, B., Weimuth, B. and Gabby, K.H., 1989, The aldo-keto reductase superfamily. CDNAS and deduced amino acid sequences of human aldehyde and aldose reductase, J. Biol. Chem. 264:9547.PubMedGoogle Scholar
  2. Carper, D., Nishimura, C., Shinohara, T., Dietzehold, B., Wistow, G., Craft, C., Kador, P. and Kinoshita J.H., 1987, Aldose reductase and p-crystallin belong to the same protein superfamily as aldehyde reductase, FEBS Lett. 220:209. Felsted, R.L. and Bachur, N.R., 1980, Mammalian carbonyl reductases, Drug Metabolism Reviews ,11:1.Google Scholar
  3. Cromlish, J.A. and Flynn, T.G., 1983, Purification and characterization of two aldose reductase isoenzymes from rabbit muscle, J. Biol. Chem. ,258:3416.PubMedGoogle Scholar
  4. Davidson, W.S. and Flynn, T.G., 1979, A functional arginine residue in NADPH -dependent aldehyde reductase from pig kidney, J. Biol. Chem. 254:3724.PubMedGoogle Scholar
  5. Flynn, T.G., Gallerneault, C., Ferguson, D., Cromlish, J.A. and Davidson, W.S., 1981, Studies on the active site of pig kidney aldehyde reductase, Biochem. Soc. Trans. 9:273.PubMedGoogle Scholar
  6. Flynn, T.G. and Cromlish, J.A., 1985, Pig brain aldehyde reductases, Prog. Clin. Biol. Res. 174:265.PubMedGoogle Scholar
  7. Flynn, T.G., Charington, B., Lyons, C., Chao, H., Hyndman, D. and Morjana, N., 1989, Chemical modification of aldehyde and aldose reductase by pyridoxal-5 ’-phosphate, Prog. Clin. Biol. Res. ,290:251.PubMedGoogle Scholar
  8. Foster, M. and Harrison, J.H., 1974, Characterization of porcine malate dehydrogenase II. Amino acid sequence of a peptide containing the active center histidine residue. Biochim. Biophys. Acta. ,351:295.PubMedGoogle Scholar
  9. Haider, A.B. and Crabbe, M J.C., 1985, Chemical modification studies on purified bovine lens aldose reductase, Ophthalmic Res. ,17:185.CrossRefGoogle Scholar
  10. Itabe, H., Sato, S. and Kador, P.F., 1989, Similarities and differences in the inhibition of rat lens aldose reductase and kidney aldehyde reductase by aldose reductase inhibitors. Invest. Ophthalmol. Vis. Sci. 30:193.Google Scholar
  11. Kador, P.F. and Sharpless, N.E., 1983, Pharmacophor requirements of the aldose reductase inhibitor site. Mol. Pharmacol. ,24:521.PubMedGoogle Scholar
  12. Kador, P.F., 1988, The role of aldose reductase in the development of diabetic complications, Medical Research Review ,8:325.CrossRefGoogle Scholar
  13. Kador, P.F., Akagi, Y., Takahashi, Y., Ikebe, H., Wyman, M. and Kinoshita, J.H., 1990, Prevention of retinal vessel changes associated with diabetic retinopathy in galactose -fed dogs by aldose reductase inhibitors. Arch. Ophthalmol. ,in press.Google Scholar
  14. Morjana, N.A., Lyons, C. and Flynn, T.G., 1989, Aldose reductase from human psoas muscle: Affinity labeling of an active lysine by pyridoxal 5 ’-phosphate and pyridoxal 5 ’-diphospho-5 ’-adenosine, J. Biol. Chem. 264:2912.PubMedGoogle Scholar
  15. Nakayama, T., Tanimoto, T. and Kador, P.F., 1989, Human erythrocyte aldose reductase, Prog. Clin. Biol. Res. ,290:265.PubMedGoogle Scholar
  16. O ’Brien, M.M., Schofield, P.J. and Edwards, M.R., 1982, Inhibition of human brain aldose reductase, J. Neurochem. ,39:810.CrossRefGoogle Scholar
  17. Poulsom, R., 1986, Inhibition of hexonate dehydrogenase and aldose reductase from bovine retina by sorbinil, Statil and valproate. Biochem. Pharmacol. ,35:2955.CrossRefGoogle Scholar
  18. Sato, S., Kador, P.F. and Kinoshita, J.H., 1988, Rat kidney aldehyde reductase: Purification and comparison with rat lens aldose reductase, in: Polyol Pathway and its Role in Diabetic Complications, N. Sakamoto, J.H. Kinoshita, P.F. Kador, and N. Hotta, eds, Excepta Medica, Elsevier Science Publishers B.V., Amsterdam. pp.72-81.Google Scholar
  19. Sato, S. and Kador, P.F., 1989, Rat lens aldehyde reductase, Invest. Ophthalmol. Vis. Sci., 30:1618.PubMedGoogle Scholar
  20. Sato, S., 1990, Polyol formation in rat kidney, inU.S.Japan Aldose Reductase Workshop, N. Hotta, ed., in press.Google Scholar
  21. Sato, S. and Kador, P.F., 1990a, Inhibition of aldehyde reductase by aldose reductase inhibitors, Biochem. Pharmacol ,in press.Google Scholar
  22. Sato, S. and Kador, P.F., 1990b, NADPH-dependent reductases in the dog lens, Exp. Eye Res. ,50:629.PubMedCrossRefGoogle Scholar
  23. Shiono, T., Sato, S., Reddy, V.N., Kador, P.F. and Kinoshita, J.H., 1987, Rapid purification of rat lens aldose reductase, Prog. Clin. Biol. Res. ,232:317.PubMedGoogle Scholar
  24. Srivastava, S.K., Petrash, J.M., Sdana, I.J., Ansari, N.H. and Partridge, C.A., 1982, Susceptibility of aldehyde and aldose reductases of human tissues to aldose reductase inhibitors, Curr. Eye Res. ,2:407.PubMedCrossRefGoogle Scholar
  25. Tanimoto T., Kador P.F., 1989, Purification of aldose and aldehyde reductases from EHS tumor cells. Biochem Pharmacol 39:445.CrossRefGoogle Scholar
  26. Terada, T., Niwase, N., Shinagawa, K., Koyama,I., Hosomi, S. and Mizoguchi, T., 1989, Bovine liver cytosolic aldehyde reductase and carbonyl reductase. Purification and characterization, Prog. Clin. Biol. Res. 290:293.PubMedGoogle Scholar
  27. Wermuth, B., Munch, J.D.B. and von Wartburg, J.-P., 1977, Purification and Properties of NADPH-dependent aldehyde reductase from human liver, J. Biol. Chem. 252:3821.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Tadashi Mizoguchi
    • 1
  • Hiroyuki Itabe
    • 1
  • Peter F. Kador
    • 1
  1. 1.National Eye InstituteNational Institutes of HealthBethesdaUSA

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