Rat Class 3 Aldehyde Dehydrogenase: Crystals and Preliminary Analysis
Class 3 aldehyde dehydrogenase (A1DH) from rat liver has been expressed in native form by E. coli from the pTALDH vector (Harper et al., 1988). This A1DH differs substantially from the class 1 and 2 AlDHs both in catalytic properties and in primary and quaternary structure. However, secondary structural predictions suggest that the subunit tertiary structures of all three A1DH classes are largely similar (Lindahl and Evces, 1984; Hempel et al., 1989). Although functional residues have been identified from chemical modification and sequence comparisons, no tertiary structure of an A1DH has yet been determined.
KeywordsAldehyde Dehydrogenase Secondary Structural Prediction Functional Residue 25mM Potassium Phosphate 10mM Sodium Acetate
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