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Rat Class 3 Aldehyde Dehydrogenase: Crystals and Preliminary Analysis

  • John Hempel
  • John P. Rose
  • Ingrid Kuo
  • Ronald Lindahl
  • Bi-Cheng Wang
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 284)

Abstract

Class 3 aldehyde dehydrogenase (A1DH) from rat liver has been expressed in native form by E. coli from the pTALDH vector (Harper et al., 1988). This A1DH differs substantially from the class 1 and 2 AlDHs both in catalytic properties and in primary and quaternary structure. However, secondary structural predictions suggest that the subunit tertiary structures of all three A1DH classes are largely similar (Lindahl and Evces, 1984; Hempel et al., 1989). Although functional residues have been identified from chemical modification and sequence comparisons, no tertiary structure of an A1DH has yet been determined.

Keywords

Aldehyde Dehydrogenase Secondary Structural Prediction Functional Residue 25mM Potassium Phosphate 10mM Sodium Acetate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Harper, K., Jones, D. E., Brennan, M. D., Lindahl, R., 1988. Characterization of a functional recombinant rat liver aldehyde dehydrogenase: Expression as a non-fusion protein in E. coli. Biochem. Biophys. Res. Comm. 152:940.PubMedCrossRefGoogle Scholar
  2. Hempel, J., Harper, K., Lindahl, R., 1989. Inducible class 3 aldehyde dehydrogenase from rat hepatocellular carcinoma and 2,3,7,8-tetrachlorodibenzo-p-dioxin-treated liver: Distant relationship to the Class 1 and 2 enzymes from mammalian liver cytosol/mitochondria. Biochemistry28:1160.PubMedCrossRefGoogle Scholar
  3. Hempel, J., Nicholas, H., Jörnvall, H., 1990. Thiol proteases and aldehyde dehydrogenases: Evolution from a common thiolesterase precursor? Proteins: Structure Function and Genetics(submitted).Google Scholar
  4. Lindahl, R., Evces, S., 1984. Aldehyde dehydrogenase II characterization of inducible isoenzymes. J. Biol. Chem. 259:11991–11996.PubMedGoogle Scholar
  5. Rose, J. P., Hempel, J., Kuo, I, Lindahl, R., Wang, B.-C., 1990. Preliminary crystallographic analysis of Class 3 rat liver aldehyde dehydrogenase. Proteins: Structure. Function and Genetics(in press).Google Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • John Hempel
    • 1
  • John P. Rose
    • 2
  • Ingrid Kuo
    • 1
  • Ronald Lindahl
    • 3
  • Bi-Cheng Wang
    • 2
  1. 1.Department of Molecular Genetics and BiochemistryUniversity of Pittsburgh School of MedicinePittsburghUSA
  2. 2.Departments of Crystallography and Biological SciencesUniversity of PittsburghPittsburghUSA
  3. 3.Department of Biochemistry and Molecular BiologyUniversity of South DakotaVermillionUSA

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