Alteration of Benzo(A)Pyrene-DNA Adduct Formation by Rats Exposed to Simple Mixtures

  • S. Stephen Bentivegna
  • Charlotte M. Witmer
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 283)


Testing the potential toxicity of mixtures is a difficult task compounded by the fact that very little is generally known about the constituents of most complex mixtures. New applications of existing methods are needed to investigate the potential adverse health effects of both simple and complex mixtures. Our laboratory has been investigating the effects of mixtures on the formation and persistence of adducts on DNA by a known toxicant along with resultant changes in metabolic enzymes as a means of studying the effects of mixtures. This approach is based on the fact that most multi-component and simple environmental mixtures have many components in common, and common effects may thus allow prediction of actions of mixtures.


Glutathione Transferase Adduct Level Mercapturic Acid Micrococcal Nuclease Aryl Hydrocarbon Hydroxylase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Habig, W.H., Pabst, M.J., Jakoby, W.B. (1974). Glutathione S-transferases: The first step in mercapturic acid formation. Jour. Biol. Chem. 249, 7130.Google Scholar
  2. Reddy, M.V., and Randerath, K. (1986). Nuclease P1-mediated enhancement of sensitivity of 32P-postlabeling test for structurally diverse DNA adducts. Carcinogenesis 7, 1543.CrossRefPubMedGoogle Scholar
  3. Reddy, M.V., and Randerath, K. (1987). 32P analysis of DNA adducts in somatic and reproductive tissues of rats treated with the anticancer antibiotic, mitomycin. Mutat. Res. 179, 75.CrossRefPubMedGoogle Scholar
  4. Robertson, I.G.C., Jensson, H., Mannervik, B., Jernstrom, B. (1986). Glutathione transferases in rat lung: the presence of transferase 7–7, highly efficient in the conjugation of glutathione with the carcinogenic (+)-benzo(a)pyrenediolepoxide. Ccwcinogenesis 7, 295.CrossRefGoogle Scholar
  5. Springer, D.L., Mann, D.B., Dankovic, D.A., Thomas, B.L., Wright, C.W., Mahlum, D.D. (1989). Influences of complex organic mixtures on tumor-initiating activity, DNA binding and adducts of benzo(a)pyrene. Ccwcinogenesis 10, 131.CrossRefGoogle Scholar
  6. Van Cantfort, J., De Graeve, J., Gielen, J.E. (1977). Radioactive assay for aryl hydrocarbon hydroxylase. Improved method and biological importance. Biochem. Biophys. Res. Comm. 79, 505.CrossRefPubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • S. Stephen Bentivegna
    • 1
  • Charlotte M. Witmer
    • 1
  1. 1.Joint Graduate Program in Toxicology/UMDNJRobert Wood Johnson Medical SchoolPiscatawayUSA

Personalised recommendations