Further Evidence for the Role of Myeloperoxidase in the Activation of Benzo[A]Pyrene-7,8-Dihydrodiol by Polymorpho-Nuclear Leukocytesm
The carcinogenic initiating activity of benzo(a)pyrene has been attributed to the generation of a stereospecific bay region diolepoxide of benzo(a)pyrene-7,8-dihydrodiol (BP)diol), anti-diolepoxide 2 (Conney, 1982). Through a myeloperoxidase (MPO)- mediated reaction, polymorphonuclear leukocytes (PMNs) can activate BP-diol to a chemiluminescent dioxetane derivative and a genotoxic electrophilic intermediate, presumably BP-diolepoxide (Trush et al., 1985; Kensler et al., 1987). This study examines the ability of PMNs or MPO to generate diolepoxides from (±)BP-diol as well as the relationship between the oxidative capability of PMNs and their ability to activate BP-diol.
KeywordsPolycyclic Aromatic Hydrocarbon Polymorphonuclear Leukocyte Oxidative Capability Human PMNs Ultimate Carcinogen
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