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Denitrosation of N-Nitrosodimethylamine in the Rat in Vivo

  • Anthony J. Streeter
  • Raymond W. Nims
  • Pamela R. Sheffels
  • Larry K. Keefer
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 283)

Abstract

The potent hepatocarcinogen N-nitrosodimethylamine (NDMA) has been shown to be metabolized by liver microsomes from acetone-induced rats via two pathways (Figure 1). While the denitrosation pathway has been studied fairly extensively in vitro (Keefer et al., 1987b; Wade et al., 1987; Amelizad et al., 1988), only a few tentative observations about its course in the intact animal have so far appeared in the literature (Heath and Dutton, 1958; Keefer et al., 1987a; Magee et al., 1988). The easiest and most direct assessment of denitrosation in vivo should come from measuring the extent of conversion of NDMA to MA, if the NDMA is labeled with carbon-14 so that the metabolically produced MA can be differentiated from the endogenous material that is known to be present (Davis and de Ropp, 1961).

Keywords

Total Radioactivity Barium Hydroxide Deuterium Isotope Effect Unchanged Compound Endogenous Material 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Anthony J. Streeter
    • 1
  • Raymond W. Nims
    • 1
  • Pamela R. Sheffels
    • 1
  • Larry K. Keefer
    • 1
  1. 1.Chemistry Section Laboratory of Comparative CarcinogenesisNational Cancer Institute Frederick Cancer Research FacilityFrederickUSA

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