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Primaquine-Induced Oxidative Stress in Isolated Hepatocytes as a Result of Reductive Activation

  • José M. Silva
  • Peter J. O’Brien
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 283)

Abstract

Primaquine,6-methoxy-8-(4-amino-l-methylbutylamino)quinoline, is one of the most effective antimarial agents but because of its low therapeutic index its use is limited. Unlike the 4-aminoquinolines it is believed to act on the liver schizont stage and not the erythrocyte stage of the parasites life cycle. However the drug is reported to cause hemolytic anemia, especially in individuals whose erythrocytes are deficient in glucose-6-phosphate dehydrogenase (Beutler, 1969). In erythrocytes, primaquine or its liver metabolite 5-OH-primaquine is reported to cause intraerythrocytic superoxide and hydrogen peroxide formation, methemoglobinemia, the formation of Heinz bodies, and occasionally the induction of hemolysis (Cohen and Hochstein, 1964; Summerfield and Tudhope, 1978).

Keywords

Redox Cycling Pulse Radiolysis Parasite Life Cycle Heinz Body Live Metabolite 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations used:

GSH

reduced glutathione

GSSG

oxidised glutathione

Hepes

4-(2- hydroxyethyl)-1-piperazine-ethane-sulphonic acid

HPLC

high performance liquid chromatography

DETAPAC

Diethylenetriamine penta-acetic acid

H2O2

hydrogen peroxide.

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References

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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • José M. Silva
    • 1
  • Peter J. O’Brien
    • 1
  1. 1.Faculty of PharmacyUniversity of TorontoTorontoCanada

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