Primaquine-Induced Oxidative Stress in Isolated Hepatocytes as a Result of Reductive Activation
Primaquine,6-methoxy-8-(4-amino-l-methylbutylamino)quinoline, is one of the most effective antimarial agents but because of its low therapeutic index its use is limited. Unlike the 4-aminoquinolines it is believed to act on the liver schizont stage and not the erythrocyte stage of the parasites life cycle. However the drug is reported to cause hemolytic anemia, especially in individuals whose erythrocytes are deficient in glucose-6-phosphate dehydrogenase (Beutler, 1969). In erythrocytes, primaquine or its liver metabolite 5-OH-primaquine is reported to cause intraerythrocytic superoxide and hydrogen peroxide formation, methemoglobinemia, the formation of Heinz bodies, and occasionally the induction of hemolysis (Cohen and Hochstein, 1964; Summerfield and Tudhope, 1978).
KeywordsRedox Cycling Pulse Radiolysis Parasite Life Cycle Heinz Body Live Metabolite
4-(2- hydroxyethyl)-1-piperazine-ethane-sulphonic acid
high performance liquid chromatography
Diethylenetriamine penta-acetic acid
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