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Expression of a cDNA Encoding Rat Liver DT-Diaphorase in Escherichia Coli

  • Qiang Ma
  • Regina Wang
  • Anthony Y. H. Lu
  • Chung S. Yang
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 283)

Abstract

Quinones and their precursors are widely distributed in nature, both as natural compounds and as environmental pollutants (Smith,1985). The toxicity and mutagenicity of quinones are believed to be due to their enzymatic one electron reduction by flavoenzymes to form semiquinones which can alkylates critical nucleophiles or generate superoxide anion radical through a redox cycling process (Thor et al., 1985; Chesis et al., 1984). DT-diaphorase (NAD(P)H: quinone oxidoreductase, EC 1.6.99.2.) is a unique flavoprotein in that it catalyzes the obligatory two electron reduction of quinones to hydroquinones via a pathway bypassing the formation of semiquinone; thus, playing a protective role against quinone toxicity (Ernster et al., 1986; Atallah et al., 1988).

Keywords

Cell Free Extract HindIII Site Quinone Oxidoreductase Quinone Reductase Electron Reduction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Qiang Ma
    • 1
  • Regina Wang
    • 2
  • Anthony Y. H. Lu
    • 2
  • Chung S. Yang
    • 1
  1. 1.Joint Graduate Program in Toxicology and Department of Chemical Biology & Pharmacognosy College of PharmacyRutgers UniversityPiscatawayUSA
  2. 2.Department of Animal & Exploratory Drug MetabolismMerck Sharp & Dohme Research LaboratoriesRahwayUSA

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