Measurement of Styrene-Oxide Cysteine Adducts in Hemoglobin by Selective Catalytic Reduction
Alkylating agents, either direct-acting or generated in vivo, form an important class of environmental mutagens and carcinogens. These agents are characterized by their ability to bind covalently to nucleophilic sites in proteins and DNA. Due to the long life time of red blood cells and the stability of protein adducts, Ehrenberg and coworkers first (Calleman 1978; Osterman-Golkar 1976) proposed the use of hemoglobin (Hb) as a dosimeter for alkylation in vivo.
KeywordsSelective Catalytic Reduction Styrene Oxide Raney Nickel Protein Adduct Glutathione Conjugate
Unable to display preview. Download preview PDF.
- Calleman, C. J., Ehrenberg, L., Jansson, B., Osterman-Golkar, S., Segerback, D., Svensson, K. and Wachtmeister, C.A. (1978). Monitoring and risk assessment by means of alkyl groups in hemoglobin in persons occupationally exposed to ethylene oxide. J. Environ. Pathol. Toxicol. 2, 427–442.PubMedGoogle Scholar
- Pettit, G.R.A.V.T., E.E. (1962). Desulfurization with Raney nickel. Organic Reactions 12, 356–529.Google Scholar
- Pizey, J. S. (1974). Raney nickel. Synthetic Reagents II, 177–288.Google Scholar