Advertisement

Comparison of 8-Hydroxydeoxyguanosine and 5-Hydroxymethyluracil as Products of Oxidative DNA Damage

  • David W. Potter
  • Zora Djuric
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 283)

Abstract

In vitro experiments with calf thymus DNA demonstrated that both 8-hydroxydeoxyguanosine (8OHdG) and 5-hydroxymethyluracil (5OHmU) are formed by H202 and Fe2+ in a concentration dependent fashion. Mannitol completely inhibited 8OHdG while 50HmU formation was only partially inhibited. Equimolar concentrations of EDTA and Fe2+ stimulated adduct formation. Singlet oxygen, generated in reaction mixtures containing methylene blue and light, resulted in formation of both 8OHdG and 5OHmU.

Keywords

Methylene Blue Singlet Oxygen Potassium Phosphate Equimolar Concentration Reactive Oxygen Intermediate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ames, B.N. (1983). Dietary carcinogens and anticarcin ogens. Oxygen radicals and degenerative diseases. Science 221, 1256–1264.CrossRefPubMedGoogle Scholar
  2. Beland, F.A., Fullerton, N.F., and Heflich, R.H. (1984). Rapid isolation, hydrolysis and chromatography of formaldehyde-modified DNA. J. Chromatogr. 308, 121–131.CrossRefPubMedGoogle Scholar
  3. Dizdaroglu, M., and Bertold, D.S. (1986). Characterization of free radical-induced base damage in DNA at biologically relevant levels. Anal. Biochem. 156, 18 2188.Google Scholar
  4. Floyd, R.A., Watson, J.J., Wong, P.K., Altmiller, D.H., and Rickard, R.C. (1986). Hydroxyl free radical adduct of deoxyguansine: sensitive detection and mechanisms of formation. Free Rad. Res. Comms. 1, 163–172.Google Scholar
  5. Floyd, R.A., West, M.S., Eneff, K.L., and Schneider, J.E. (1989). Methylene blue plus light mediates 8-hyroxyguanine formation in DNA. Arch. Biochem. Biophys. 273, 106–111.Google Scholar
  6. Giorgio, M., and Aust, S.D. (1989). The role of iron in oxygen radical mediated lipid peroxidation. Chem.-Biol. Interactions 71, 1-19.Google Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • David W. Potter
    • 1
  • Zora Djuric
    • 2
  1. 1.Rohm and Haas Co.Spring HouseUSA
  2. 2.Dept. of Internal MedicineWayne State UniversityDetroitUSA

Personalised recommendations