Generation of Free Radicals Results in Increased Rates of Protein Degradation in Human Erythrocytes
The hydrazines represent a class of agents well recognized for their potent hemolytic activity in red blood cells. Phenylhydrazine, one of the most potent hemolyzing agents known, is one of the few agents capable of producing hemolysis in vitro. Phenylhydrazine reacts with the heme of oxyhemoglobin to generate free radical species which alkylate the porphyrin ring system (Saito and Itano, 1981; Augusto et al., 1982; Winterbourn and French, 1977; Ortiz de Montellano et al., 1983). Similar oxidation reactions have been reported for other substituted hydrazines and alkyl hydrazines (Winterbourn and French, 1977; Ortiz de Montellano et al., 1983). The organic hydroperoxides (cumene hydroperoxide, t-butyl hydroperoxide) also decompose to yield organic free radicals capable of producing protein damage (Trotta et al., 1983; Thornalley et al., 1983; Taffe et al., 1987; Maples et al., 1990).
KeywordsHuman Erythrocyte Cumene Hydroperoxide Organic Hydroperoxide Free Radical Species Monobasic Sodium Phosphate
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