Generation of Free Radicals Results in Increased Rates of Protein Degradation in Human Erythrocytes

  • Anne M. Mortensen
  • Melissa Runge-Morris
  • Raymond F. Novak
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 283)


The hydrazines represent a class of agents well recognized for their potent hemolytic activity in red blood cells. Phenylhydrazine, one of the most potent hemolyzing agents known, is one of the few agents capable of producing hemolysis in vitro. Phenylhydrazine reacts with the heme of oxyhemoglobin to generate free radical species which alkylate the porphyrin ring system (Saito and Itano, 1981; Augusto et al., 1982; Winterbourn and French, 1977; Ortiz de Montellano et al., 1983). Similar oxidation reactions have been reported for other substituted hydrazines and alkyl hydrazines (Winterbourn and French, 1977; Ortiz de Montellano et al., 1983). The organic hydroperoxides (cumene hydroperoxide, t-butyl hydroperoxide) also decompose to yield organic free radicals capable of producing protein damage (Trotta et al., 1983; Thornalley et al., 1983; Taffe et al., 1987; Maples et al., 1990).


Human Erythrocyte Cumene Hydroperoxide Organic Hydroperoxide Free Radical Species Monobasic Sodium Phosphate 
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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Anne M. Mortensen
    • 1
    • 2
  • Melissa Runge-Morris
    • 2
  • Raymond F. Novak
    • 1
    • 2
  1. 1.The Institute of Chemical ToxicologyWayne State UniversityDetroitUSA
  2. 2.The Department of Molecular BiologyNorthwestern University Medical SchoolChicagoUSA

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