Morphological Cell Transformation and DNA Adduction by Benz(J)Aceanthrylene and its Presumptive Reactive Metabolites in C3H10T1/2CL8 Cells
Benz(j)aceanthrylene [B(j)A], a cyclopenta-fused polycyclic aromatic hydrocarbon found in coal combustion emissions [Grimmer et al., 1985; Schmidt et al., 1986], is a strong inducer of morphological transforming activity in C3H10T1/2CL8 (C3H10T1/2) mouse embryo fibroblasts [Mohapatra et al., 1987]. The metabolism of B(j)A by these cells has been studied and the results differ from those found with Aroclor-1254 induced rat liver S9. In C3H10T1/2 cells the major metabolite is 9,10dihydro-9,10-dihydroxy-B(j)A with minor amounts of 1,2-dihydro-1,2-dihydroxy-B(j)A [Mohapatra et al., 1987], while in rat liver S9 the major metabolite is 1,2-dihydro-1,2-dihydroxy-B(j)A [Nesnow et al., 1988]. These results suggest that while the major route of metabolic activation by Aroclor-1254 induced rat liver S9 is through B(j)A-1,2-oxide, the arene oxide at the cyclopenta-ring, metabolic activation of B(j)A in C3H10T1/2 cells might be via two routes: botharene oxide [B(j)A-1,2-oxide] formation and bay-region diol-epoxide formation [9,10-dihydro-9,10-dihydroxy-B(j)A-7,8-oxide]. This study was undertaken to clarify the role of the putative reactive intermediates, 9,10-dihydro-9,10-dihydroxy-B(j)A-7,8-oxide [B(j)A-diol-epoxide], and B(j)A-1,2-oxide in the metabolic activation of B(j)A in C3H10T1/2 cells.
KeywordsPolycyclic Aromatic Hydrocarbon Mouse Embryo Fibroblast Grand Island Biological Arene Oxide Mouse Embryo Fibroblast Cell
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