In Vivo Hemoglobin Thiyl Radical Formation as a Consequence of Hydrazine-Based Drug Metabolism
For a variety of reasons, the possibility of free radical metabolism has not received much attention in the past, although Michaelis of the Michaelis-Menten equation was interested in free radical metabolites and their importance in biochemistry in the 1930’s. One reason for the late development of this area is that most biochemicals, as opposed to aromatic drugs and industrial chemicals, are not easily metabolized through free radical intermediates. Another reason is that unless something can be demonstrated with a whole animal, there will always be some question of its actual existence in biology. To detect something as ephemeral as a free radical formed inside a whole animal is certainly not easy. In such investigations, the toxic effects of free radicals are presumably the result of purely chemical reactions; therefore, a knowledge of the radical’s chemistry under physiological conditions is necessary.
KeywordsSpin Adduct Radical Adduct Free Radical Intermediate Free Radical Metabolism Nitroxide Free Radical
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