Neuropeptide mRNA Expression in Human Basal Ganglia
We have examined the distribution in human basal ganglia of cells expressing mRNAs for several neuropeptides in control and diseased postmortem material by in situ hybridization histochemistry using 32P-labelled oligonucleotides as hybridization probes. In control cases, enkephalin mRNA showed a patchy-like distribution. Cells containing somatostatin mRNA and neuropeptide Y mRNA were scattered throughout the caudate and putamen in a very similar pattern. Cholecystokinin mRNA was not found in the striatum. In Parkinson’s disease, our preliminary results showed a decrease in the hybridization signal with the enkephalin probe. In contrast, somatostatin mRNA levels and neuropeptide Y levels did not exhibit significant changes. In Huntington’s chorea, we have observed a marked decrease in the levels of enkephalin mRNA in both caudate and putamen but not in the nucleus accumbens. In contrast, no alteration of the hybridization signal was observed for both somatostatin and neuropeptide Y mRNAs. These results show that in situ hybridization histochemistry is very useful for the study of the molecular anatomy of the human basal ganglia and their pathology.
KeywordsBasal Ganglion Nucleus Accumbens Hybridization Signal Postmortem Delay Hybridization Histochemistry
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