Organization of Cholinergic Perikarya in the Caudate Nucleus of the Cat with Respect to Heterogeneities in Enkephalin and Substance P Staining
We present here a series of studies examining the organization of cholinergic perikarya in the caudate nucleus of the cat and their relationship to heterogeneities observed in enkephalin and substance P staining. Cholinergic interneurons comprise only a small proportion of the total number of striatal cells (Phelps et al., 1985). Most studies have not reported any obvious organization in the distribution of these cells in single sections (Fibiger, 1982) although some authors have commented that they did not appear to be evenly distributed but were organized into poorly defined clusters (Everitt et al., 1988; Mesulam et al., 1984). In addition, no consistent relationship has been demonstrated between the cholinergic perikarya and the striosomal compartment as delineated by acetylcholinesterase. While cholinergic neuropil and the high affinity choline uptake system are distributed primarily within the matrix (Graybiel et al., 1986; Rhodes et al., 1987), cholinergic cell bodies have been reported to occur both within patch and matrix in equal densities (Brand, 1980) or to be slightly increased in density within the matrix compartment (Graybiel et al., 1983, 1986).
KeywordsCaudate Nucleus Cholinergic Neuron Dorsal Striatum Cholinergic Cell Striatal Cell
Unable to display preview. Download preview PDF.
- Beckstead, R. M., 1987, Striatal substance P cell clusters coincide with the high density terminal zones of the discontinuous nigrostriatal dopaminergic projection system in the cat: A study by combined immunohistochemistry and autoradiographic axon-tracing, Neuroscience. 20: 557.PubMedCrossRefGoogle Scholar
- Bolam, J. P., Ingham, C. A., Izzo, P. N., Levey, A. I., Rye, D. B., Smith, A. D. and Wainer, B.H.,1986, Substance P-containing terminals in synaptic contact with cholinergic neurons in the neostriatum and basal forebrain: A double immunocytochemical study in the rat, Brain Res., 397: 279.Google Scholar
- Bolam, J. P. and Izzo, P. N., 1987, Possible sites of transmitter interaction in the neostriatum: An anatomical approach, in Sandler, M., Feuerstein, C. and Scatton, B. (eds) Neurotransmitter interactions in the basal ganglia, New York: Raven Press, p. 47.Google Scholar
- Graybiel, A. M., Chesselet, M.-F., Wu, J.-Y., Eckenstein, F. and Joh, T. E., 1983, The relation of striosomes in the caudate nucleus of the cat to the organization of early developing dopaminergic fibers, GAD-positive neuropil, and CAT-positive neurons, Soc. Neurosci. Abstr., 9: 14.Google Scholar
- Graybiel, A. M., Ragsdale, C. W., Yoneoka, E. S. and Elde, R. P., 1981, An immunohistochemical study of enkephalins and other neuropeptides in the striatum of the cat with evidence that the opiate peptides are arranged to form mosaic patterns in register with the striosomal compartments visible by acetylcholinesterase staining, Neuroscience, 6: 377.PubMedCrossRefGoogle Scholar
- Martone, M., Young, S. J., Armstrong, D. M. and Groves, P. M., 1989, Ultrastructural examination of enkephalin and substance P input to cholinergic neurons in the rat neostriatum. Soc. Neurosci. Abstr. 15: 911.Google Scholar
- Mesulam, M.-M., Mufson, E. J., Levey, A. I. and Wainer,B. H., 1984, Atlas of cholinergic neurons in the forebrain and upper brainstem of the macaque based on monoclonal choline acetyltransferase immunohistochemistry and acetylcholinesterase histochemistry, Neuroscience. 3: 669.CrossRefGoogle Scholar
- Penny, G. R., Afsharpour and Kitai, S. T.,1986, The glutamate decarboxylase, leucine enkephalin-, methionine enkephalin-and substance P-immunoreactive neurons in the neostriatum of the rat and cat: Evidence for partial population overlap, Neuroscience. 17:1011.Google Scholar
- Rhodes, K. J., Joyce, J. N., Sapp, D. W. and Marshall, J. F., 1987, [3H] Hemicholinium3 binding in rabbit striatum: Correspondence with patchy acetylcholinesterase staining and a method for quantifying striatal compartments, Brain Res., 412: 400.Google Scholar