Correlated Light and Electron Microscopy of Golgi-Impregnated Neostriatal Neurons after 6-Hydroxydopamine Lesions in the Rat
The removal of the dopamine input to the neostriatum by 6-hydroxydopamine lesions is a useful method of studying possible neuronal responses which may occur in the neostriatum in Parkinson’s disease. We have shown that medium size spiny neurons have a lower spine density in the neostriatum which has lost its dopamine input compared with the control unlesioned neostriatum (Ingham et al., 1989). This result persists to the same degree at relatively short and long time periods after the lesion. The present study expands on these results and takes the same material to the electron microscope for ultrastructural evaluation.
KeywordsSpine Density Ultrastructural Evaluation Medium Size Spiny Neuron Asymmetrical Synaptic Contact Lower Spine Density
Unable to display preview. Download preview PDF.
- Bolam J.P. and Ingham C.A., 1990. Combined morphological and histochemical techniques for the study of neuronal microcircuits. “Handbook of Chemical Neuroanatomy, Volume 8, Neuronal Microcircuits – Combined morphological, immunocytochemical and electrophysiological techniques for the study of synaptic interactions between identified CNS neurons.” Van den Pol A. and Wouterlood F. eds, Elsevier, Amsterdam.Google Scholar
- Lindefors N., Brodin E., Tossman U., Segovia J. and Ungerstedt U., 1989. Tissue levels and in vivo release of tachykinins and GABA in striatum and substantia nigra of rat brain after unilateral striatal dopamine denervation. Exp. Brain Res. 74; 527–534.Google Scholar
- Schultz W., 1982. Depletion of dopamine in the striatum as an experimental model of parkinsonism: direct effects and adaptive mechanisms. Prog. Neurobiol. 18; 121–166.Google Scholar
- Ungerstedt U., 1971. Postsynaptic supersenstivity after 6- hydroxydopamine induced degeneration of the nigrostriatal dopamine system. Acta physiol. scand. Suppl. 367; 69–93.Google Scholar