Use of 13C and 17O NMR to Study Wheat Starch-Water-Sugar Interactions with Increasing Temperatures

  • D. Sobczynska
  • C. Setser
  • H. Lim
  • L. Hansen
  • J. Paukstelis
Part of the Basic Life Sciences book series (BLSC, volume 56)


Nuclear magnetic resonance (NMR) is a very sensitive probe of molecular motion in the fluid state. NMR spectroscopic techniques reported in this overview have been used to study starch-water-sugar systems at the molecular level. The increased onset temperature of starch gelatinization in the presence of sugars is well known, but not so well understood. Several mechanisms and variations of those mechanisms have been suggested including 1) a competition between the sugars and starch for the available water and thus changes in the free water volume (Derby et al., 1975; Hoseney et al., 1977; Slade and Levine, 1988b), 2) an inhibition of starch swelling by the sugars (D’Appolonia, 1972; Bean and Yamazaki, 1978; Savage and Osman, 1978; Wooton and Bamunuarachchi, 1980; Lelievre, 1984), which might be related to the competition for water, and 3) a penetration of the starch granule by the sugars and interactions leading to a stabilization of the granule that requires more energy to disrupt (Spies and Hoseney, 1982). Studies of the starch-sugar-water systems have used the amylograph for macro-level measurements of viscosity changes (Lund, 1984), microscopic techniques to observe the loss of birefringence and increased swelling (Bean and Osman, 1959; Watson, 1977; Bean and Yamazaki, 1978; Bean et al., 1978), and differential scanning calorimetry (DSC) to measure melting temperatures and enthalpies (Wooton and Bamunuarachchi, 1980; Spies and Hoseney, 1980).


Relaxation Rate Starch Granule Onset Temperature Sucrose Concentration Water Mobility 
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  1. Bean, M. M. and Osman, E. M., 1959, Behavior of starch during food preparation. II. Effects of different sugars on the viscosity and gel strength of starch pastes. Food Res., 24:665.Google Scholar
  2. Bean, M. M. and Yamazaki, W. T., 1978, Wheat starch gelatinization in sugar solutions. I. Sucrose; light microscopy and viscosity effects. Cereal Chem., 55:936.Google Scholar
  3. Bean, M. M., Yamazaki, W. T. and Donelson, D. H., 1978, Wheat starch gelatinization in sugar solutions. II. Fructose, glucose, and sucrose cake performance. Cereal Chem., 55:945.Google Scholar
  4. Bock, K. and Lemieux, R., 1982, The conformational properties of sucrose in aqueous solution: Intramolecular hydrogen-bonding. Carbohydr. Res., 100:63.CrossRefGoogle Scholar
  5. Callaghan, P. T., Jolley, K. W., Lelievre, J. and Wong, R. B. K., 1983, Nuclear magnetic resonance studies of wheat starch pastes. J. Colloid and Interface Sci., 92:332.CrossRefGoogle Scholar
  6. D’Appolonia, B. L., 1972, Effect of bread ingredients on starch gelatinization properties as measured by the amylograph. Cereal Chem., 49:532.Google Scholar
  7. Derby, R. I., Miller, B. S., Miller, B. F. and Trimbp, H. B., 1975, Visual observations of wheat-starch gelatinization in limited water systems. Cereal Chem., 52:702.Google Scholar
  8. Duckworth, R. B., 1981, in: “Water activity influences on food quality,” (Rockland, L. B., Stewart, G. F., ed.) p. 295–317. Academic Press., Inc.Google Scholar
  9. Halle, B., Anderson, T., Forsen, S. and Lindman, B., 1981, Protein hydration from water oxygen-17 magnetic relaxation. J. Am. Chem. Soc, 103:500.CrossRefGoogle Scholar
  10. Halle, B. and Wennerstrom, H., 1981, Interpretation of magnetic resonance data from water nuclei in heterogenous systems. J. Chem. Phys., 75:1928.CrossRefGoogle Scholar
  11. Hansen, L. M., Paukstelis, J. V. and Setser, C. S., 1987, C nuclear magnetic resonance spectroscopic methods for investigating sucrose-starch interactions with increasing temperature. Cereal Chem., 64:449.Google Scholar
  12. Hansen, L. M., Setser, C. S., and Paukstelis, J. V., 1989, Investigations 13 of sugar-starch interactions using 13C-NMR. I. Sucrose. Cereal Chem., 66:411.Google Scholar
  13. Hoseney, R. C., Atwell, W. A. and Lineback, D. R., 1977, Scanning electron microscopy of starch isolated from baked products. Cereal Foods World., 22:56.Google Scholar
  14. Hull, W. E., 1982, Two-Dimensional NMR. Bruker Analytische Messtechack., Karlsruhe, West Germany.Google Scholar
  15. Lechert, H. T., 1981, in: “Water Activity Influences on Food quality,” L. B. Rockland and G. F. Stuart, (Ed.), pp. 223–245. Academic Press, Inc., New York.Google Scholar
  16. Lelievre, J., 1984, Effects of sugars on the swelling of crosslinked potato starch. J. Colloid and Interface Sci., 101:225.CrossRefGoogle Scholar
  17. Leung, H. K., Magnuson, J. A. and Bruinsma, B. L., 1979, Pulsed nuclear magnetic resonance study of water mobility in flour doughs. J. Food Sci., 44:1408.CrossRefGoogle Scholar
  18. Lund, D., 1984, Influence of time, temperature, moisture, ingredients, and processing conditions on starch gelatinization. CRC Crit. Rev. in Food Sci. and Nutr., 20:249.CrossRefGoogle Scholar
  19. Richardson, S. J., Baianu, I. C. and Steinberg, M. P., 1986, Mobility of water in wheat flour suspensions as studied by proton and oxygen-17 nuclear magnetic resonance. J. Agr. and Food Chem., 34:17.CrossRefGoogle Scholar
  20. Richardson, S. J., Baianu, I. C. and Steinberg, M. P., 1987a, Mobility of water in starch-sucrose systems determined by deuterium and oxygen-17 NMR. Starke, 39:302.CrossRefGoogle Scholar
  21. Richardson, S. J., Steinberg, M. P., De Vor, R. E. and Sutherland J. W., 1987b, Characterization of the oxygen-17 nuclear magnetic resonance water mobility response surface. J. Food Sci., 52:189.CrossRefGoogle Scholar
  22. Richardson, S. J., Baianu, I. C. and Steinberg, M. P., 1987c, Mobility of water in sucrose solutions determined by deuterium and oxygen-17 nuclear magnetic resonance measurements. J. Food Sci., 52:806.CrossRefGoogle Scholar
  23. Savage, H. L. and Osman, E. M., 1978, Effects of certain sugars and sugar alcohols on the swelling of corn starch granules. Cereal Chem., 55:447.Google Scholar
  24. Shiotsubo, T. and Takahashi, K., 1984, Differential thermal analysis of potato starch gelatinization. Biol. Chem., 48:9.Google Scholar
  25. Slade, L. and Levine, H., 1988a, Non-equilibrium melting of native granular starch: Part I. Temperature location of the glass transition associated with gelatinization of A-type cereal starchs. Carbohy. Polymers, 8:183.CrossRefGoogle Scholar
  26. Slade, L. and Levine, H., 1988b, Recent advances in starch retrograda-tion, in: “Recent Developments in Industrial Polysaccharides,” S. S. Stivala, V. Crescenzi, and I.C.M. Dea (Ed.), Gordon and Breach Science, New York. In press.Google Scholar
  27. Spies, R. D. and Hoseney, R. C, 1982, Effect of sugars on starch gelatinization. Cereal Chem., 59:128.Google Scholar
  28. Sterling, C., 1964, Starch-primulin fluorescence. Protoplasma, 59:180.CrossRefGoogle Scholar
  29. Wooton, M. and Bamunuarachchi, A., 1980, Application of differential scanning calorimetry to starch gelatinization. III. Effect of sucrose and sodium chloride. Starke 32:126.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • D. Sobczynska
  • C. Setser
    • 1
  • H. Lim
    • 1
  • L. Hansen
    • 1
  • J. Paukstelis
    • 2
  1. 1.Department of Foods and NutritionKansas State UniversityManhattanUSA
  2. 2.Department of ChemistryKansas State UniversityManhattanUSA

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