In Vivo Phosphorus NMR Studies of the Hepatic Metabolism of Amino Sugars
Although the amino sugars glucosamine and galactosamine occur naturally and, as N-acetyl derivatives, are constituents of various cellular glycocon-jugates, the administration of large doses can result in significant toxicity. This aspect of their metabolism has led to evaluations of these compounds as potential chemotherapeutic agents (Quastel and Cantero, 1953; Bekesi et al., 1969; Keppler et al., 1985), and to studies aimed at elucidating the mechanisms involved in the production of hepatocellular injury (Decker and Keppler, 1974, 1972). As with a number of other cellular toxins, the toxicity results from biochemical activation or “lethal synthesis” (Decker and Keppler, 1972; Shull et al., 1966), since the metabolic transformations involved begin with phosphorylation by the corresponding enzymes of glucose and galactose metabolism (Ballard, 1966; Walker and Khan, 1968; Oguchi et al., 1977). This metabolism leads ultimately to uridylate trapping effects and, in the case of galactosamine, to the production of metabolites which are not observed in untreated tissue.
KeywordsDifference Spectrum Amino Sugar Nucleoside Triphosphate Hexose Phosphate Phosphate Monoester
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