Uptake, Metabolism, and Storage of Phosphate and Nitrogen in Plant Cells; an NMR Perspective
A number of Nuclear Magnetic Resonance (NMR) Spectroscopy techniques can be used to study pH regulation and various aspects of nutrient metabolism in plant material. In this study phosphorus-31 NMR has been used to determine the energy state (ATP) and the intracellular cytoplasmic and vacuolar pH of cultured plant cells and algae. For the algae it was found that the chemical shift of the terminal polyphosphate resonance provided a good monitor of the vacuolar pH which was estimated at pH 5.5. A cytoplasmic pH of 7.2 was determined from the chemical shifts of the Pi and glucose-6-phosphate resonances. Phosphate uptake could also be followed by 31P NMR and these studies showed that Pi was stored as polyphosphates in algae, but as vacuolar Pi in certain higher plants such as Catharanthus roseus and Nicotiana tabacum.
KeywordsAmide Arginine Assimilation Glutamine Macromolecule
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