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NMR of Carbohydrates at the Surface of Cells

  • Harold C. Jarrell
  • Ian C. P. Smith
Part of the Basic Life Sciences book series (BLSC, volume 56)

Abstract

Carbohydrates attached to lipid and proteins are a major element of the cell surface, where, anchored in the membrane they modulate interactions with the outside world. Glycolipids may be divided into two major classes distinguished by the nature of the hydrophobic anchor. The glycolipids of bacteria and plants generally consist of a mono-or oligo-saccharide glycosidically linked to the glycerol 3-position of 1,2-diacylglycerol (Curatolo, 1987a). The major glycolipids of animals are in the second class which consist of carbohydrate glycosidically linked to ceramide glycosphingolipids (Curatolo, 1987a). Glycolipids are intimately involved with membrane structure, recognition, immune function, interaction with toxins and biological pathogens, and growth control in both normalcy and disease (Curatolo, 1987a; Thompson and Tillack, 1985; Critchley, 1979). While the important roles that these lipids play is well established, how these roles are fulfilled and influenced by the environment in which these molecules exist is far from being understood. For these reasons, there has been increasing interest in attempting to systematically delineate various physio-chemical properties of these lipids as pure systems and in mixtures (Curatolo, 1987b). The ultimate goal of such studies is to gain insight into how these parameters may influence the various biological roles that the glycolipids can assume.

Keywords

Nuclear Magnetic Resonance Spectrum Liquid Crystalline Phase Glycerol Backbone Solid State Nuclear Magnetic Resonance Bovine Pancreatic Trypsin Inhibitor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Abragam, A., 1961, The principles of nuclear magnetism, Oxford University Press, London.Google Scholar
  2. Auger, M., Carrier, D., Smith, I. C. P., and Jarrell, H. C., Elucidation of motional modes in glycoglycerolipids -a H NMR relaxation and lineshape study, J. Amer. Chem. Soc., in press.Google Scholar
  3. Auger, M., and Jarrell, H. C., Elucidation of Slow Motions in Glyco-glycerolipid Bilayers by Two-Dimensional Solid-State Deuteron NMR, Chem. Phys. Letters, in press.Google Scholar
  4. Auger, M., Smith, I. C. P., and Jarrell, H. C., unpublished results, a. Auger, M., van Calsteren, M. R., Smith, I. C. P., and Jarrell, H. C., unpublished results, b. Bloom, M., and Smith, I. C. P., 1985, Manifestations of lipid-protein interactions in deuterium NMR, in: Progress in Protein-Lipid Interactions, Watts, A., and de Pont, J., eds. Elsevier, Amsterdam, 61–88.Google Scholar
  5. Carrier, D., Giziewicz, J. B., Moir, D., Smith, I. C. P., and Jarrell, H. C, 1989, Dynamics and orientation of glycolipid headgroups by 2 H-NMR: gentiobiose, Biochim. Biophys. Acta., 983:100.PubMedCrossRefGoogle Scholar
  6. Critchley, D. R., 1979, Glycolipids as membrane receptors important in growth regulation, in: Surfaces of Normal and Malignant Cells, R.O., Hynes, Ed., John Wiley and Sons, New York.Google Scholar
  7. Curatolo, W., 1987a, Glycolipid function, Biochim. Biophys. Acta., 906:137.PubMedGoogle Scholar
  8. Curatolo, W., 1987b, The physical properties of glycolipids, Biochim. Biophys. Acta, 906:111.PubMedGoogle Scholar
  9. Davis, J. H., 1983, The description of membrane lipid conformation, order 2 and dynamics by H-NMR, Biochim. Biophys. Acta., 737:117.PubMedGoogle Scholar
  10. Jarrell, H. C., Giziewicz J. B., and Smith, I. C. P., 1986, Structure and 2 dynamics of a glyceroglycolipid: A H NMR study of head group orientation, ordering and effect on lipid aggregate structure. Biochemistry, 25:3950.CrossRefGoogle Scholar
  11. Jarrell, H. C., Jovall, P. A., Giziewicz, J. B., Turner, L. A., and Smith, I. C. P., 1987a, Determination of conformational properties 2 of glycolipid head groups by H NMR of oriented multibilayers, Biochemistry, 26:1805.PubMedCrossRefGoogle Scholar
  12. Jarrell, H. C., Wand, A. J., Giziewicz, J. B., and Smith, I. C. P., 1987b, The dependence of glyceroglycolipid orientation and dynamics on head-group structure, Biochim. Biophys. Acta., 897:69.PubMedCrossRefGoogle Scholar
  13. Jeffrey, K. R., 1981, Nuclear magnetic relaxation in a spin 1 system. Bull. Magn. Reson., 3:69.Google Scholar
  14. Meier, P., Ohmes E., and Kothe, G., 1986, Multipulse dynamic nuclear magnetic resonance of phospholipid membranes, J. Chem. Phys., 85:3598.CrossRefGoogle Scholar
  15. Renou, J. P., Giziewicz, J. B., Smith, I. C. P., and Jarrell, H. C., 1989, Glycolipid membrane surface structure: orientation, conformation, and motion of a disaccharide headgroup, Biochemistry, 28:1804.PubMedCrossRefGoogle Scholar
  16. Skarjune, R., and Oldfield, E., 1982, Physical studies of cell surface and cell membrane structure. Deuterium nuclear magnetic resonance studies of N-palmitoyl-glucosylceramide (cerebroside) head group structure, Biochemistry, 21:3154.PubMedCrossRefGoogle Scholar
  17. Smith, I. C. P., and Jarrell, H. C., 1983, Deuterium and phosphorus NMR of microbial membranes, Acc. Chem. Res., 16:266.CrossRefGoogle Scholar
  18. Thompson, T. E., and Tillack, T. W., 1985, Organization of glycosphin-golipids in bilayers and plasma membranes of mammalian cells, Ann. Rev. Biophys. Biophys. Chem., 14:361.CrossRefGoogle Scholar
  19. Wittebort, R. J., Olejniczak, E. T., and Griffin, R. G., 1987, Analysis of deuterium nuclear magnetic resonance lineshapes in anisotropic media, J. Chem. Phys., 36:5411.CrossRefGoogle Scholar
  20. Yu, R. K., Koerner, T. A. W., Demori, P. C., Scarsdale, J. N., and Prestegard, J. H., 1984, Recent advances in structural studies of ganglioside: primary and secondary structures, in: Ganglioside Structure, Function and Biomedical Potential, Leden, R. W., Yu, R. K., Rapport, M. M., and Suzuki, K., eds. Plenum Press, New York.Google Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Harold C. Jarrell
    • 1
  • Ian C. P. Smith
  1. 1.Division of Biological SciencesNational Research Council of CanadaOttawaCanada

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