Abstract
Since the introduction of the monoclonal antibody (McAb) method by Kohler and Milstein over ten years ago, a major goal of cell biologists has been to define and characterize lineage and cell type specific antigens that may allow the identification and manipulation of specific cells. While in principle it is possible to prepare McAbs to any subcellular compartment or organelle, a large amount of effort has gone into the identification of cell surface antigens. There are a number of sound reasons for this preference.
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Barton, C.H., Dickson, G., Gower, H.J., Rowett, L.H., Putt, W., Elsom, V., Moore, S.E., Goridis, C. and Walsh, F.S. 1988. Complete sequence and in vitro expression of a tissue specific phosphatidylinositol-linked N-CAM isoform from skeletal muscle. Development 104: 165–173.
Blau, H.M., Pavlath, G.K., Hardeman, E.C., Webster, S.G., Miller, S.C. and Webster, C. 1985. Plasticity of the differentiated state. Science 230: 758–766.
Covault, J., Merlie, J.P., Goridis, C. and Sanes, J.R. 1986. Molecular forms of N-CAM and its RNA in developing and denervated skeletal muscle. J. Cell Biol. 102: 731–739.
Cunningham, B.A., Hemperly, J.J., Murray, B.A., Prediger, E.A., Brackenbury, R. and Edelman, G.M. 1987. Neural cell adhesion molecule: structure immunoglobulin-like domains, cell surface modulation and alternative RNA splicing. Science 236: 799–806.
Doherty, P., Barton, C.H., Dickson, G., Seaton, P., Rowett, L.H., Moore, S.E., Gower, H.J. and Walsh, F.S. 1989. Neuronal process outgrowth of human sensory neurons on monolayers of cells transfected with cDNAs for five human N-CAM isoforms. J. Cell Biol. 109: 789–798.
Gower, H.J., Barton, C.H., Elsom, V.L., Thompson, J., Moore, S.E., Dickson, G. and Walsh, F.S. 1988. Alternative splicing generates a secreted form of N-CAM in muscle and brain. Cell 55: 955–964.
Gower, H.J., Moore, S.E., Dickson, G., Elsom, V.L., Nayak, R. and Walsh, F.S. 1989. Cloning and characterization of a myoblast cell surface antigen defined by 24.1D5 monoclonal antibody. Development 105: 723–732.
Hurko, O., McKee, L., Zuurveld, J. and Swick, H.M. 1987. Comparison of Duchenne and normal myoblasts from a heterozygote. Neurology 37: 675–681.
Moore, S.E. and Walsh, F.S. 1986. Nerve dependent regulation of N-CAM expression in skeletal muscle. Neuroscience 18: 499–505.
Moore, S.E., Thompson, J., Kirkness, V., Dickson, J.G. and Walsh, F.S. 1987. Skeletal muscle neural adhesion molecule (N-CAM): changes in protein and mRNA species during myogenesis of muscle cell lines. J. Cell. Biol. 105: 1377–1386.
Partridge, T.A., Morgan, J.E., Coulton, J.R., Hoffman, E.P. and Kunkel, L.M. 1989. Conversion of MDX myofibers from dystrophin-negative to -positive by injection of normal myoblasts. Nature 337: 176–179.
Patel, K., Moore, S.E., Dickson, G., Rossell, R.J., Beverley, P.C., Kemshead, J.T. and Walsh, F.S. 1989. Neural cell adhesion molecule (N-CAM) is the antigen recognized by monoclonal antibodies of similar specificity in small cell lung carcinoma and neuroblastoma. Int. J. Cancer (in press).
Thompson, J., Dickson, G., Moore, S.E., Gower, H.J., Putt, W., Kenimer, J.G., Barton, C.H. and Walsh, F.S. 1989. Alternative splicing of the neural cell adhesion molecule generates variant extracellular domain structure in skeletal muscle and brain. Genes and Development 3: 348–357.
Walsh, F.S. and Ritter, M.A. 1981. Surface antigen differentiation during human myogenesis in culture. Nature 289: 60–64.
Walsh, F.S., Moore, S.E., Woodroofe, M.N., Hurko, O., Nayak, R., Brown, S.M. and Dickson, J.G. 1984. Characterization of human muscle differentiation antigens. Exp. Biol. and Med. 9: 50–56.
Walsh, F.S. and Dickson, G. 1989. Generation of multiple N-CAM polypeptides from a single gene. Bioassays (in press).
Walsh, F.S., Dickson, G., Moore, S.E. and Barton, C.H. 1989. Unmasking N-CAM. Nature 339: 516.
Webster, C., Pavlath, G.K., Parks, D.R., Walsh, F.S. and Blau, H.M. 1988. Isolation of human myoblasts with the fluorescence-activated cell sorter. Exp. Cell. Res. 174: 252–265.
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© 1990 Plenum Press, New York
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Walsh, F.S. (1990). N-CAM is a Target Cell Surface Antigen for the Purification of Muscle Cells for Myoblast Transfer Therapy. In: Griggs, R.C., Karpati, G. (eds) Myoblast Transfer Therapy. Advances in Experimental Medicine and Biology, vol 280. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5865-7_7
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DOI: https://doi.org/10.1007/978-1-4684-5865-7_7
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