Myogenic Conversion of Human Non-Muscle Cells for the Diagnosis and Therapy of Neuromuscular Diseases

  • A. F. Miranda
  • T. Mongini
  • E. Bonilla
  • A. D. Miller
  • W. E. Wright
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 280)


Control of tissue-specific gene expression in eukaryotes represents one of the central unanswered questions in developmental biology. Davis et al (1) isolated a muscle regulatory cDNA called MyoD from mouse cells: when transfected into fibroblasts or adipocytes, MyoD converts these cells to myoblasts, which, under appropriate conditions, can fuse and differentiate. Later, several additional genes, myd (2), myogenin (3) and Myf-5 (4), also capable of stably converting non-muscle cells to myoblasts were isolated. The availability of these probes will lead to a better understanding of the ordered regulation of gene expression in muscle and other differentiating tissues. These factors that can produce myogenic conversion may also have practical applications in the diagnosis or even the treatment of hereditary human myopathies, such as Duchenne Muscular dystrophy (DMD). This will be the theme of this presentation.


DUCHENNE Muscular Dystrophy Duchenne Muscular Dystrophy DUCHENNE Muscular Dystrophy Patient Anterior Tibial Muscle Human Myoblast 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • A. F. Miranda
    • 1
  • T. Mongini
    • 1
    • 2
  • E. Bonilla
    • 1
  • A. D. Miller
    • 1
    • 3
  • W. E. Wright
    • 1
    • 4
  1. 1.College of Physicians and SurgeonsColumbia UniversityNew YorkUSA
  2. 2.Clinical Neurologica IIUniversity of TurinTurinItaly
  3. 3.Fred Hutchinson Cancer Research CenterSeattleUSA
  4. 4.University of Texas Southwestern Medical SchoolDallasUSA

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