Abstract
Control of tissue-specific gene expression in eukaryotes represents one of the central unanswered questions in developmental biology. Davis et al (1) isolated a muscle regulatory cDNA called MyoD from mouse cells: when transfected into fibroblasts or adipocytes, MyoD converts these cells to myoblasts, which, under appropriate conditions, can fuse and differentiate. Later, several additional genes, myd (2), myogenin (3) and Myf-5 (4), also capable of stably converting non-muscle cells to myoblasts were isolated. The availability of these probes will lead to a better understanding of the ordered regulation of gene expression in muscle and other differentiating tissues. These factors that can produce myogenic conversion may also have practical applications in the diagnosis or even the treatment of hereditary human myopathies, such as Duchenne Muscular dystrophy (DMD). This will be the theme of this presentation.
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References
R. L. Davis, H. Weintraub, and A. B. Lassar, Expression of a single transfected cDNA converts fibroblasts to myoblasts, Cell 51: 987 (1987).
D. F. Pinney, S. H. Pearson-White, S. F. Konieczny, K. E. Latham, and C. P. Emerson Jr., Myogenic lineage determination and differentiation: Evidence for a regulatory gene pathway, Cell 53: 781 (1988).
W. E. Wright, D. A. Sassoon, and V.K. Lin, Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD, Cell 56: 607 (1989).
T. Braun, G. Buschhausen-Denker, E. Bober, E. Tannich and H. H. Arnold, A novel human muscle factor related to but distinct from MyoDl induces myogenic conversion in 10Th fibroblasts, EMBO J. 8: 701 (1989).
B. T. Darras, P. Blattner, J. F. Harper, A. J. Spiro, S. Alter, and U. Francke, Intragenic deletions in 21 Duchenne muscular dystrophy (DMD)/Becker muscular dystrophy (BMD) families studied with the dystrophin cDNA: location of breakpoints on Hindlll and BglII exon-containing fragment maps, meiotic and mitotic origin of the mutations, Am. J. Hum. Genet. 43: 620 (1988).
B. T. Darras, M. Koenig, L.M. Kunkel, and U. Francke, Direct method for prenatal diagnosis and carrier detection in Duchenne-Becker muscular dystrophy using the entire dystrophin cDNA, Am. J. Med. Genet. 29: 713 (1988).
E. P. Hoffman and L. M. Kunkel, Dystrophin abnormalities in Duchenne/ Becker muscular dystrophy, Neuron 2: 1019 (1989).
S. M. Forrest, G. S. Cross, A. Speer, D. Gardner-Medwin, J. Burn, and K.E. Davies, Preferential deletion of exons in Duchenne and Becker muscular dystrophies, Nature 329: 638 (1987).
J. S. Chamberlain, R. A. Gibbs, J. E. Ranier, P. N. Nguyen, and C. T. Caskey, Deletion screening of the Duchenne muscular dystrophy locus via multiplex DNA amplification, Nucleic Acid Res. 16: 11141 (1988).
E. Bonilla, G. Samitt, A. F. Miranda, A. P. Hays, G. Salviati, S. DiMauro, L. M. Kunkel, E. P. Hoffman, and L.P. Rowland, Duchenne muscular dystrophy: deficiency of dystrophin at the muscle cell surface, Cell 54: 447 (1988).
H. Sugita, K. Arahata, T. Ishiguro, Y. Suhara, T. Tsukahara, S. Ishiura, C. Eguchi, I. Nonaka, and E. Ozawa, Negative immunostaining of Duchenne muscular dystrophy and mdx muscle surface membrane with antibody against synthetic peptide fragment predicted from DMD cDNA, Proc. Jap. Acad. (B) 64: 210 (1988).
E. E. Zubrzycka-Gaarn, D. E. Bulman, G. Karpati, A. H. M. Burghes, B. Belfall, H. J. Klamut, J. Talbot, R. S. Hodges, P. N. Ray, and R. G. Worton, The Duchenne muscular dystrophy gene product is localized in the sarcolemma of human skeletal muscle, Nature 333: 466 (1988).
M. S. Ecob-Prince, M. A. Hill, and A. E. Brown, Localization of dystrophin in cultures of human muscles, Muscle and Nerve 12: 594 (1989).
A. A. Lev, C. C. Feener, L. M. Kunkel, and R. H. Brown, Expression of the Duchenne’s muscular dystrophy gene in cultured muscle cells, J. Biol. Chem. 262: 15817 (1987).
A. F Miranda, E. Bonilla, G. Martucci, C. T. Moraes, A. P. Hays, and S. DiMauro, Immunocytochemical study of dystrophin in muscle cultures from patients with Duchenne muscular dystrophy and unaffected control patients, Am J. Pathol. 132: 410 (1988).
M. Wigler, A. Pellicer, S. Silverstein, R. Axel, G. Urlaub and L. Chasin, DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells, Proc. Natl. Acad. Sci. USA 76: 1373 (1979).
E. P. Hoffman, R. H. Brown, and L. M. Kunkel, Dystrophin: The protein product of the Duchenne muscular dystrophy locus, Cell 51: 919 (1987a).
T. D. Palmer, R. A. Hock, W. R. A. Osborne, and A. D. Miller, Efficient retrovirus-mediated transfer and expression of a human adenosine deaminase gene in diploid skin fibroblasts from an adenosine deaminase-deficient human, Proc. Natl. Acad. Sci. USA 84: 1055 (1987).
H. Weintraub, S. J. Tapscott, R. L. Davis, M. J. Thayer,M. A. Adam, A. B. Lassar, and A. D. M.ller, Activation of muscle-specific genes in pigment, nerve, fat, liver and fibroblast cell lines by forced expression of MyoD, Proc. Natl. Acad. Sci. USA. 86: 5434 (1989).
T. Mongini, A. F. Miranda, E. Bonilla, A. D. Miller, W. E. Wright, and H. Weintraub, Manuscript in preparation.
Hurko, E. P. Hoffman, L. McKee, D. R. Johns, and L. M. Kunkel, Dystrophin analysis in clonal myoblasts derived from a Duchenne muscular dystrophy carrier, Am. J. Hum. Genet. 44: 820 (1989).
A. F. Miranda, U. Francke, E. Bonilla, G. Martucci, B. Schmidt, G. Salviati, and M. Rubin, Dystrophin immunocytochemistry in muscle culture: detection of a carrier of Duchenne muscular dystrophy, Am. J. Med. Genet. 32: 268 (1989).
C. Webster, G. K. Pavlath, D. R. Parks, F. S. Walsh, and H. M. Blau, Isolation of human myoblasts with the fluorescence-activated cell sorter, Expl. Cell Res. 174: 252 (1988).
G. Karpati, Y. Pouliot, E. Zubrzycka-Gaarn, S. Carpenter, P. N. Ray, R. G. Worton, and P. Holland, Dystrophin is expressed in mdx skeletal muscle fibers after normal myoblast implantation, Am. J. Pathol. 135: 27 (1989).
T. A. Partridge, J. E. Morgan, G. R. Coulton, E. P. Hoffman and L. M. Kunkel, Conversion of mdx myofibres from dystrophin-negative to -positive by injection of normal myoblasts, Nature 337: 176 (1989).
A. F. Miranda and T. Mongini, Diseased muscle in tissue culture, in: “Myology”, A. G. Engel and B. Q. Banker, eds., McGraw Hill Book Comp., New York (1986).
H. M. Blau, C. Webster, and G. K. Pavlath, Defective myoblasts identified in Duchenne Muscular Dystrophy, Proc. Natl. Acad. Sci. USA. 80: 4856 (1983).
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© 1990 Plenum Press, New York
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Miranda, A.F., Mongini, T., Bonilla, E., Miller, A.D., Wright, W.E. (1990). Myogenic Conversion of Human Non-Muscle Cells for the Diagnosis and Therapy of Neuromuscular Diseases. In: Griggs, R.C., Karpati, G. (eds) Myoblast Transfer Therapy. Advances in Experimental Medicine and Biology, vol 280. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5865-7_23
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