Improved Media for Rapid Clonal Growth of Normal Human Skeletal Muscle Satellite Cells

  • Richard G. Ham
  • Judy A. St. Clair
  • Sarah D. Meyer
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 280)


Many investigators who use cell culture as a research tool fail to give adequate attention to culture media. Virtually all “standard” nutrient media were published more than 20 years ago, and many widely used formulations are at least 30 years old. Thus, the culture medium is often the most obsolete and neglected component of the entire experimental system. This presentation describes recent improvements in cell culture media for human muscle satellite cells (HMSC) and seeks to encourage their use.


Satellite Cell Clonal Growth DUCHENNE Muscular Dystrophy Muscle Satellite Cell Human Microvascular Endothelial Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Allen, R. E., and Boxhorn, L. K., 1989, Regulation of skeletal muscle satellite cell proliferation and differentiation by transforming growth factor-beta, insulin-like growth factor I, and fibroblast growth factor. J. Cell. Physiol. 138: 311.PubMedCrossRefGoogle Scholar
  2. Blau, H. M., and Webster, C., 1981, Isolation and characterization of human muscle cells. Proc. Natl. Acad. Sci. USA 78: 5623.CrossRefGoogle Scholar
  3. Blau, H. M., Webster, C., and Pavlath, G. K., 1983, Defective myoblasts identified in Duchenne muscular dystrophy. Proc. Natl. Acad. Sci USA 80: 4856.PubMedCrossRefGoogle Scholar
  4. Florini, J. R., 1987, Hormonal control of muscle growth. Musc. Nerve 10: 577.CrossRefGoogle Scholar
  5. Ham, R. G., 1963, An improved nutrient solution for diploid Chinese hamster and human cell lines. Exp. Cell Res. 29: 515.PubMedCrossRefGoogle Scholar
  6. Ham, R.G., and St. Clair, J.A., 1989, Differentiation and partial maturation of human skeletal muscle cells in serum-free culture. In Vitro Cell. Dev. Biol. 25: 44A.CrossRefGoogle Scholar
  7. Ham, R. G., St. Clair, J. A., Webster, C., and Blau, H. M., 1988, Improved media for normal human muscle satellite cells: serum-free clonal growth and enhanced growth with low serum. In Vitro Cell. Dev. Biol. 24: 833.PubMedCrossRefGoogle Scholar
  8. Hauschka, S. D., 1974, Clonal analysis of vertebrate myogenesis. II. Environmental influences on human muscle differentiation. Dev. Biol. 37: 329.CrossRefGoogle Scholar
  9. Hauschka, S. D., and Konigsberg, I. R., 1966, The influence of collagen on the development of muscle clones. Proc. Natl. Acad. Sci. USA 55: 119.PubMedCrossRefGoogle Scholar
  10. Knedler, A., and Ham, R. G., 1987, Optimized medium for clonal growth of human microvascular endothelial cells with minimal serum. In Vitro Cell. Dev. Biol. 23: 481.PubMedCrossRefGoogle Scholar
  11. Konigsberg, I. R., 1963, Clonal analysis of myogenesis. Science 140: 1273.PubMedCrossRefGoogle Scholar
  12. Meyer, S.D., St. Clair, J.A., and Ham, R.G., 1989, EGF is a mitogen for human skeletal muscle satellite cells (HMSC) but does not block their differentiation. J. Cell Biol. 109: 305a.Google Scholar
  13. Webster, C., Pavlath, G. K., Parks, D. R., Walsh, F. S., and Blau, H. M., 1988, Isolation of human myoblasts with the fluorescence-activated cell sorter. Exp. Cell. Res. 174: 252.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Richard G. Ham
    • 1
  • Judy A. St. Clair
    • 1
  • Sarah D. Meyer
    • 1
  1. 1.Department of Molecular, Cellular and Developmental BiologyUniversity of ColoradoBoulderUSA

Personalised recommendations