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Myoblasts, Satellite Cells, and Myoblast Transfer

  • Frank E. Stockdale
  • E. Janet Hager
  • Susan E. Fernyak
  • Joseph X. DiMario
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 280)

Abstract

The prospects for introducing “foreign” nuclei through the cell fusion process have been considered since it first became clear that skeletal muscle fibers form by fusion of many mononucleated myoblasts rather than the proliferation of skeletal muscle nuclei within fibers (Stockdale and Holtzer, 1961). While cell fusion is cell type specific, there is no restriction in myoblast fusion across species, as skeletal myogenic cells from different species or classes of vertebrates are capable of adding nuclei to forming fibers (Yaffe and Feldman, 1965). The introduction of nuclei by PEG-mediated cell fusion has made it feasible to introduce a limited number of nuclei of non-myogenic cells into fibers in cell culture (Blau, 1983). However, specificity of cell recognition and fusion limits the introduction of new nuclei into existing fibers or into newly forming fibers to those cells that have been committed to a myogenic fate. The prospects of converting non-myogenic cells to myogenic cells by the transfection of commitment or determination genes broadens the repertoire of cells that could be used for contributing nuclei to muscle fibers using normal fusion mechanisms (Konieczny et al., 1986; Lassar et al., 1986; Weintraub et al., 1989).

Keywords

Satellite Cell Skeletal Muscle Fiber Myogenic Cell Nuclear Domain Slow Myosin Heavy Chain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Blau, H. M., 1983, Cytoplasmic activation of human nuclear genes in stable heterocaryons, Cell, 32: 1171.PubMedCrossRefGoogle Scholar
  2. Blau, H. M., 1988, Hierarchies of regulatory genes may specify mammalian development, Cell, 53: 673.PubMedCrossRefGoogle Scholar
  3. Blau, H. M., Pavlath, G. K., Hardeman, E. C., Chiu, C.-P., Silberstein, L., Webster, S. G., Miller, S. C., and Webster, C., 1985, Plasticity of the differentiated state, Science, 230: 758.PubMedCrossRefGoogle Scholar
  4. Feldman, J. L., and Stockdale, F. E., 1988, Commitment to formation of distinct myoutube types in chicken satellite cells, J. Cell. Biochem.Google Scholar
  5. Hall, Z. W., and Ralston, E., 1989, Nuclear domains in muscle cells, Cell, 59, 771.PubMedCrossRefGoogle Scholar
  6. Kelly, A. M., and Zacks, S. I., 1969, The histogenesis of rat intercostal muscle, J. Cell Biol., 42: 135.PubMedCrossRefGoogle Scholar
  7. Konieczny, S. F., Baldwin, A. S., and Emerson, C. P., Jr., 1986, Myogenic determination and differentiation of 10T1/2 cell lineages: evidence for a simple genetic regulation system, in: “Molecular Biology of Muscle Development,” Alan R. Liss, Inc., New York, 21.Google Scholar
  8. Lassar, A. B., Paterson, B. M., and Weintraub, H., 1986, Transfection of a DNA locus that mediates the conversion of 10T1/2 fibroblasts to myoblasts, Cell, 47: 649.PubMedCrossRefGoogle Scholar
  9. Miller, J. B., and Stockdale, F. E., 1986a, Developmental origins of skeletal muscle fibers: Clonal analysis of myogenic cell lineages based on fast and slow myosin heavy chain expression, Proc. Natl. Acad. Sci. USA, 83: 3860.Google Scholar
  10. Miller, J. B., and Stockdale, F. E., 1986b, Developmental regulation of the multiple myogenic cell lineages of the avian embryo, J. Cell Biol., 103: 2197.PubMedCrossRefGoogle Scholar
  11. Miller, J. B., and Stockdale, F. E., 1989, Multiple cellular processes regulate expression of slow myosin heavy chain isoforms during avian myogenesis in vitro, Dev. Biol., 139: 393.CrossRefGoogle Scholar
  12. Miller, J. B., Crow, M. T., and Stockdale, F. E., 1985, Slow and fast myosin heavy chain content defines three types of myotubes in early muscle cell cultures, J. Cell Biol., 101: 1643.PubMedCrossRefGoogle Scholar
  13. Mintz, B., and Baker, W. W., 1967, Normal mammalian muscle differentiation and gene control of isocitrate dehydrogenase synthesis, Proc. Natl. Acad. Sci. USA, 58: 592.PubMedCrossRefGoogle Scholar
  14. Nicolas, J.-F., and Bonnerot, C., 1987, Recombinant retrovirus, cell lineage and gene expression in the mouse embryo, in: “Cellular Factors in Development and Differentiation - Embryos, Teratocarcinomas and Differentiated Tissues,” Alan R. Liss, Inc., New York, 1.Google Scholar
  15. Partridge, T. A., Morgan, J. E., Coulton, G. R., Hoffman, E. P., and Kunkel, L. M., 1989, Conversion of mdx myofibres from dystrophin-negative to -positive by injection of normal myoblasts, Nature, 337: 176.PubMedCrossRefGoogle Scholar
  16. Pavlath, G. K., and Blau, H. M., 1986, Expression of muscle genes in heterokaryons depends on gene dosage, J. Cell Biol., 102: 124.PubMedCrossRefGoogle Scholar
  17. Pavlath, G. K., Rich, K., Webster, S. G., and Blau, H. M., 1989, Localization of muscle gene products in nuclear domains, Nature, 337: 570.Google Scholar
  18. Ralston, E., and Hall, Z. W., 1989, Transfer of a protein by a single nucleus to nearby nuclei in multinucleated myotubes, Science, 244: 1066.PubMedCrossRefGoogle Scholar
  19. Rao, P.N, and Johnson, R.T., 1970, Mammal cell fusion: Studies on the regulation of DNA synthesis and mitosis, Nature 225: 159.PubMedCrossRefGoogle Scholar
  20. Salviati, G., Biasia, E., and Aloisi, M., 1986, Synthesis of fast myosin induced by fast ectopic innervation of rat soleus muscle is restricted to the ectopic endplate region, Nature, 322: 637.PubMedCrossRefGoogle Scholar
  21. Sanes, J. R., 1989, Analyzing cell lineage with a recombinant retrovirus, Trends Neurosci., 12: 21.PubMedCrossRefGoogle Scholar
  22. Schafer, D. A., Miller, J. B., and Stockdale, F. E., 1987, Cell diversification within the myogenic lineage: In vitro generation of two types of myoblasts from a single myogenic progenitor cell, Cell, 48: 659.Google Scholar
  23. Silberstein, L., Webster, M., Travis, M., and Blau, H. M., 1986, Developmental progression of myosin gene expression in cultured muscle cells, Cell, 46: 1076.CrossRefGoogle Scholar
  24. Stockdale, F. E., and Holtzer, H., 1961, DNA synthesis and myogenesis, Exp. Cell Res., 24: 508.PubMedCrossRefGoogle Scholar
  25. Stockdale, F. E., and Miller, J. B., 1987, The cellular basis of myosin heavy chain isoform expression during development of avian skeletal muscles, Dev. Biol., 123: 1.Google Scholar
  26. Stockdale, F. E., 1989, Skeletal muscle fiber specification during development and the myogenic lineage, in: “The Assembly of the Nervous System,” L. Landmesser, ed., Alan R. Liss, Inc., New York, 37.Google Scholar
  27. Stockdale, F. E., Miller, J. B., Feldman, J. L., Lamson, G., and Hager, J., 1989, Myogenic cell lineages: Commitment and modulation during differentiation of avian muscle, in: Cellular and Molecular Biology of Muscle Development,“ L. Kedes and F. E. Stockdale, eds., Alan R. Liss, Inc., New York, 3.Google Scholar
  28. Stockdale, F. E., Miller, J. B., Schafer, D. A., and Crow, M. T., 1986, Myosins, myotubes, and myoblasts. Origins of fast and slow muscle fibers, in: “Molecular Biology of Muscle Development,” C. Emerson, D. Fischman, B. Nadal-Ginard, M. A. Q. Siddiqui, eds., Alan R. Liss, Inc., New York, 29: 213.Google Scholar
  29. Weintraub, H., Tapscott, S. J., Davis, R. L., Thayer, M. J., Adam, M. A., Lassar, A. B., and Miller, A. D., 1989, Activation of muscle-specific genes in pigment, nerve, fat, liver, and fibroblast cell lines by forced expression of MyoD, Proc. Natl. Acad. Sci. USA, 86: 5434.Google Scholar
  30. Yaffe, D., and Feldman, M., 1965, The formation of hybrid multinucleated muscle fibers from myoblasts of different genetic origin, Dev. Biol., 11: 300.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Frank E. Stockdale
    • 1
  • E. Janet Hager
    • 1
  • Susan E. Fernyak
    • 1
  • Joseph X. DiMario
    • 1
  1. 1.Stanford School of MedicineStanfordUSA

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