Practical Aspects of Myoblast Implantation: Investigations on Two Inherited Myopathies in Animals

  • Jennifer E. Morgan
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 280)


For some years, our group has been studying the idea of implanting normal muscle precursor cells (mpc) into the skeletal muscles of individuals suffering from inherited myopathies, in an attempt to alleviate the disease. The idea behind this is simple: multinucleated muscle fibres are formed during muscle regeneration by the fusion of mononucleated mpc (Stockdale and Holtzer, 1961); during growth, mpc become incorporated into the muscle fibres (Moss and Leblond, 1971). It follows that if we can implant normal mpc into growing or regenerating myopathic muscle, the implanted mpc would become incorporated into the host muscle fibres (Watt et al. 1982, 1984). The presence of myonuclei of normal origin, carrying the normal genome, within the myopathic fibre should help to alleviate the myopathy.


Duchenne Muscular Dystrophy Extensor Digitorum Longus Duchenne Muscular Dystrophy Phosphorylase Kinase Muscle Precursor Cell 


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  1. Bulfield, G. Siller, W.G., Wight P.A.L. and Moore, K.J. (1984). X-chromosome-linked muscular dystrophy (mdx) in the mouse. Proc. Natl. Acad. Sci. U.S.A. 81, 1189–1192.PubMedCrossRefGoogle Scholar
  2. Cardasis, C.A. and Cooper, G.W. (1975). An analysis of nuclear numbers in individual muscle fibres during differentiation and growth: a satellite cell-muscle fibre growth unit. J. Exp. Zool. 191, 333–346.PubMedCrossRefGoogle Scholar
  3. Cohen, P.T.W., Burchell, A. and Cohen, P. (1976). The molecular basis of skeletal muscle phosphorylase kinase deficiency. Eur. J. Biochem. 66, 347–356.PubMedCrossRefGoogle Scholar
  4. Coulton, G.R., Morgan, J.E., Partridge, T.A. and Sloper, J.C. (1988 a). The mdx mouse skeletal muscle myopathy: I. A histological, morphometric and biochemical investigation. Neuropathol. appl. Neurobiol. 14, 53–70.PubMedCrossRefGoogle Scholar
  5. Coulton, G.R., Curtin, N.A., Morgan, J.E., and Partridge, T.A. (1988 b). The mdx mouse skeletal myopathy: II. Contractile properties. Neuropathol. appl. Neurobiol. 14, 299–314.PubMedCrossRefGoogle Scholar
  6. Hoffman, E.P., Brown, R.H. and Kunkel, L.M. (1987). Dystrophin: the protein product of the Duchenne Muscular Dystrophy locus. Cell 51, 919–928.PubMedCrossRefGoogle Scholar
  7. Law, P.K.,Goodwin, T.G. and Wang, M.G. (1988). Normal myoblast injections provide genetic treatment for murine dystrophy. Muscle and Nerve 11, 525–533.PubMedCrossRefGoogle Scholar
  8. Moss, F.P. and Leblond, C.P. (1971). Satellite cells as the source of nuclei in muscles of growing rats. Anat. Rec. 170, 421–436.PubMedCrossRefGoogle Scholar
  9. Morgan, J.E., Coulton, G.R. and Partridge, T.A. (1987). Muscle precursor cells invade and repopulate freeze-killed grafts. J. Muscle Res. Cell Motil. 8, 386–396.PubMedCrossRefGoogle Scholar
  10. Morgan, J.E. (1988). Phosphorylase kinase activities in damaged mouse muscle. J. Neurol. Sci. 86, 149–158.PubMedCrossRefGoogle Scholar
  11. Morgan, J.E., Watt, D.J., Sloper, J.C. and Partridge, T.A. (1988). Partial correction of an inherited biochemical defect of skeletal muscle by grafts of normal muscle precursor cells. J. Neurol. Sci. 86, 137–147.PubMedCrossRefGoogle Scholar
  12. Partridge, T.A., Morgan, J.E., Coulton, G.R., Hoffman, E.P. and Kunkel, L.M. (1989). Conversion of mdx myofibres from dystrophin-negative to -positive by injection of normal myoblasts. Nature 337, 176–179.PubMedCrossRefGoogle Scholar
  13. Stockdale, F.E. and Holtzer, H. (1961). DNA synthesis and myogenesis. Exp. Cell Res. 24, 508–520.PubMedCrossRefGoogle Scholar
  14. Stockdale, F.E. and Holtzer, H. (1961). DNA synthesis and myogenesis. Exp. Cell Res. 24, 508–520.PubMedCrossRefGoogle Scholar
  15. Watt, D.J., Lambert, K., Morgan, J.E., Partridge, T.A. and Sloper, J.C. (1982). Incorporation of donor muscle precursor cells into an area of regeneration in the host mouse. J. Neurol. Sci. 57, 319–331.PubMedCrossRefGoogle Scholar
  16. Watt, D.J., Morgan, J.E. and Partridge, T.A. (1984). Use of mononuclear precursor cells to insert allogeneic genes into growing mouse muscles. Muscle and Nerve 7, 741–750.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Jennifer E. Morgan
    • 1
  1. 1.Department of HistopathologyCharing Cross and Westminster Medical SchoolLondonUK

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