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Evaluation of Immunoassays for Electron Microscopy

  • Frances W. Doane
  • Nan Anderson
  • Francis Lee
  • Kathryn Pegg-Feige
  • John Hopley

Abstract

In recent years immunoelectron microscopy (IEM) has become one of the several immunoassays available to virologists for detection and identification of viruses. Like other immunological assays, the reliability depends on careful attention to test conditions (e.g. antigen/antibody concentration, incubation time, temperature, pH) and to a regard for incorporation of a range of appropriate controls. This chapter will discuss several IEM techniques that can be applied to fluid specimens commonly encountered in diagnostic virology. Consideration will be given to some of the variables that affect the efficiency of these tests, and to the advantages and disadvantages of IEM relative to other immunoassays. Additional practical details are available from several published sources (Doane, 1974, 1986, 1987, 1988a, 1988b; Doane and Anderson, 1977, 1987; Katz and Kohn, 1984; Kjeldsberg, 1986).

Keywords

Virus Particle Tobacco Mosaic Virus Immunoelectron Microscopy Rubella Virus Agar Diffusion Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • Frances W. Doane
    • 1
  • Nan Anderson
    • 1
  • Francis Lee
    • 1
  • Kathryn Pegg-Feige
    • 1
  • John Hopley
    • 1
  1. 1.Department of MicrobiologyUniversity of TorontoTorontoCanada

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