Advertisement

Detection of a Highly Conserved Region of Herpesviridae DNA by In Vitro Enzymatic Amplification: Application to the Detection of a New Human Herpesvirus

  • Steven Wolinsky
  • Jan Andersson
  • Anne Rowley
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 278)

Abstract

The human herpesviruses, which include herpes simplex virus types 1 and 2 (HSV-1 and HSV-2), cytomegalovirus, Epstein-Barr virus, and varicella-zoster virus, cause infections which have significant morbidity and mortality (1–3). Established methods used for diagnosis of infection with these viruses are direct virus isolation from clinical specimens, detection of viral antigens or antibodies, or in some instances clinical criteria (1–3). Conventional culture techniques for some of these viruses require several weeks for a positive result to be detected by characteristic cytopathic effect. In certain clinical situations, virus that can grow rapidly in culture may not be detected because of the low viral titer (4). Serologic tests and antigen detection methods may not be positive at an early time point when antiviral therapy would be efficacious. Furthermore, an antibody response may be delayed or absent in the immunocompromised host, where early diagnosis is imperative (5). Rapid detection of the virus would allow the institution of early antiviral therapy and obviate the need for invasive diagnostic procedures.

Keywords

Bacterial Meningitis Human Herpesvirus Herpes Simplex Encephalitis Enzymatic Amplification Herpes Encephalitis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Nahmias, A. J., Keyserling, H. and Lee, F. K. (1989) in Viral Infections of Humans, Epidemiology and Control, ed. Evans, A. S. ( Plenum, New York).Google Scholar
  2. 2.
    Evans, A. F. and Niederman, J. C. (1989) in Viral Infections of Humans, Epidemiology and Control, ed. Evans, A. S. ( Plenum, New York).CrossRefGoogle Scholar
  3. 3.
    Ho, M. (1982) Cytomegalovirus, Biology and Infection ( Plenum, New York).Google Scholar
  4. 4.
    Nahmias, A. J., Whitley, R. J., Visintine, A. N., Takei, Y., Alford, C. A., Jr. and Collaborative Antiviral Study Group. (1982) J. Infect. Dis. 145, 829–836.PubMedCrossRefGoogle Scholar
  5. 5.
    Rasmussen, L., Keisall, D., Nelson, R., Carney, W., Hirsch, M., Winston, D., Preiksaitis, J. and Merigan, T. C. (1982) J. Infect. Dis. 145, 191–199.PubMedCrossRefGoogle Scholar
  6. 6.
    Saiki, R. K., Scharf, S. J., Faloona, F., Mullis, K. B., Horn, G. T., Erlich, H. A. and Arnheim, N. (1985) Science (Washington, D.C., 1883-) 230, 1350–1354.CrossRefGoogle Scholar
  7. 7.
    Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B. and Erlich, H. A. (1988) Science (Washington, D.C, 1883-) 239, 487–491.CrossRefGoogle Scholar
  8. 8.
    Kwok, S., Mack, D. H., Mullis, K. B., Poiesz, B., Ehrlich, G., Blair, D., Friedman-Kien, A. and Sninsky, J. J. (1987) J. Virol. 61, 1690–1694.PubMedGoogle Scholar
  9. 9.
    Manos, M. M., Ting, Y., Wright, D. K., Lewis, A. J., Broker, T. R. and Wolinsky, S. M. (1989) Cancer Cells, Vol. 7, Molecular Diagnostics of Human Cancer (Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y. ), pp. 209–214.Google Scholar
  10. 10.
    Shibata, D., Martin, W. J., Appleman, M. D., Causey, D. M., Leedom, J. M. and Arnheim, N. (1988) J. Infect. Dis. 158, 1185–1192.PubMedCrossRefGoogle Scholar
  11. 11.
    Wong, S. W., Wahl, A. F., Yuan, P., Arai, N., Pearson, B. E., Arai, K., Korn, D., Hunkapiller, M. W. and Wang, P. S. F. (1988) EMBO J. 7, 37–47.PubMedGoogle Scholar
  12. 12.
    Ecker, J. R. and Hyman, R. W. (1982) Proc. Natl. Acad Sci. USA 79, 156–160.Google Scholar
  13. 13.
    Gibbs, J. S., Chiou, H. C., Hall, J. D., Mount, D. W., Retondo, M. J., Weller, S. K. and Coen, D. M. (1985) Proc. Natl. Acad Sci. USA 82, 7969–7973.Google Scholar
  14. 14.
    Earl, P. L., Jones, E. V. and Moss, B. (1986) Proc. Natl. Acad Sci. 83, 3659–3663.PubMedCrossRefGoogle Scholar
  15. 15.
    Saiki, R. K., Bugawan, T. L., Horn, G. T., Mullis, K. B. and Erlich, H. A. (1986) Nature (London) 324, 163–166.CrossRefGoogle Scholar
  16. 16.
    Wolinsky, S. M., Rinaldo, C. R., Kwok, S., Sninsky, J. J., Gupta, P., Imagawa, D., Farzadegan, H., Jacobson, L. P., Grovit, K. S., Lee, M. H., Chmiel, J. S., Ginsburg, H., Kaslow, R. A. and Phair, J. P. (1989) Ann. Intern. Med 111, 961–972.PubMedGoogle Scholar
  17. 17.
    Maniatis, T., Fritsch, E. F., Sambrook, J. (1982) in Molecular Cloning: A Laboratory Manual, eds. Maniatis, Fritsch, and Sambrook ( Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y. ), pp. 382–389.Google Scholar
  18. 18.
    Larder, B. A., Kemp, S. D. and Darby, G. (1987) EMBO J. 6, 169–175.PubMedGoogle Scholar
  19. 19.
    Argos, P. (1988) Nucleic Acids Res. 16, 9909–9916.PubMedCrossRefGoogle Scholar
  20. 20.
    Parker, A. C., Craig, J. I. O., Collins, P., Oliver, N. and Smith, J. (1987) Lancet 2, 1461.PubMedCrossRefGoogle Scholar
  21. 21.
    Schnipper, L. E. and Crumpacker, C. S. (1980) Proc. Nati Acad Sci. 77, 2270–2273.CrossRefGoogle Scholar
  22. 22.
    Mack, D. H. and Sninsky, J. J. (1988) Proc. Nati Acad Sci. USA 85, 6977–6981.Google Scholar
  23. 23.
    Demmler, G. J., Buffone, G. J., Schimbor, C. M. and May, R. A. (1988) J. Infect. Dis. 158, 1177–1184.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Steven Wolinsky
    • 1
  • Jan Andersson
    • 3
  • Anne Rowley
    • 2
  1. 1.Department of MedicineNorthwestern University Medical SchoolChicagoUSA
  2. 2.Department of PediatricsNorthwestern University Medical SchoolChicagoUSA
  3. 3.Department of ImmunologyStockholm UniversityStockholmSweden

Personalised recommendations